LOCUS       sequence-61929-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Burrows AE, Smogorzewska A, Elledge SJ
  TITLE     Polybromo-associated BRG1-associated factor components BRD7 and 
            BAF180 are critical regulators of p53 required for induction of 
            replicative senescence.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14280-5. doi: 
            10.1073/pnas.1009559107. Epub 2010 Jul 26.
  PUBMED    20660729
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1009559107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-08-10- 10"; volume: "107"; issue: "32"; pages: "14280-5"
COMMENT     Sequence Label: MSCV N-term HA-FLAG puro BRD7 shRescue
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..71
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             84..110
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    126..150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatctgc caccatggac tacaaggatg
       61 acgatgacaa gctcgatgga ggatacccat acgatgttcc agattacgct gctcgaggtt
      121 atcaaacaag tttgtacaaa aaagcaggct tcatgggcaa gaagcacaag aagcacaagt
      181 cggacaaaca cctctacgag gagtatgtag agaagccctt gaagctggtc ctcaaagtag
      241 gagggaacga agtcaccgaa ctctccacgg gcagctcggg gcacgactcc agcctcttcg
      301 aagacaaaaa cgatcatgac aaacacaagg acagaaagcg gaaaaagaga aagaaaggag
      361 agaagcagat tccaggggaa gaaaagggga gaaaacggag aagagttaag gaggataaaa
      421 agaagcgaga tcgagaccgg gtggagaatg aggcagaaaa agatctccag tgtcacgccc
      481 ctgtgagatt agacttgcct cctgagaagc ctctcacaag ctctttagcc aaacaagaag
      541 aagtagaaca gacacccctt caagaagctt tgaatcaact gatgagacaa ttgcagagaa
      601 aagatccaag tgctttcttt tcatttcctg tgactgattt tattgctcct ggctactcca
      661 tgatcattaa acacccaatg gacttctcca cgatgaagga aaagatcaag aacaatgact
      721 atcagtccat agaagaacta aaggataact tcaaactaat gtgtactaat gccatgattt
      781 acaataaacc agagaccatt tattataaag ctgcaaagaa gctgttgcac tcaggaatga
      841 aaattcttag ccaggaaaga attcagagcc tgaagcagag catagacttc atggctgact
      901 tgcagaaaac tcgaaagcag aaagatggaa cagacacctc acagagtggg gaggacggag
      961 gctgctggca
//
