LOCUS       sequence-61948-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-TCTT
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(606..622)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(606..622)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(619..641)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    630..646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(654..660,661..678,679..684))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    699..720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(837..854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaattgggcc ctctagatgc atgctcgagc ggccgccagt gtgatggata tctgcagaat
       61 tcgcccttat atagatgccg tcctagcgcg tctcctgacc ccagagcagg tcgtggccat
      121 tgcctcgaat ggagggggca aacaggcgtt ggaaaccgta caacgattgc tgccggtgct
      181 gtgccaagcg cacggactca ccccagagca ggtcgtggcg atcgcaagcc acgacggagg
      241 aaagcaagcc ttggaaacag tacagaggct gttgcctgtg ctgtgccaag cgcacggcct
      301 caccccagag caggtcgtgg ccattgcctc gaatggaggg ggcaaacagg cgttggaaac
      361 cgtacaacga ttgctgccgg tgctgtgcca agcgcacgga ttaaccccag agcaggtcgt
      421 ggccattgcc tcgaatggag ggggcaaaca ggcgttggaa accgtacaac gattgctgcc
      481 ggtgctgtgc caagcgcacg gcttaagaga cgtgggcaca ggaaagatac ttaagggcga
      541 attccagcac actggcggcc gttactagtg gatccgagct cggtaccaag cttggcgtaa
      601 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      661 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      721 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      781 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      841 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      901 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      961 ggccagcaaa aaggccagga accgta
//
