LOCUS       sequence-61956-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-TCTC
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(613..631)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(614..631)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(649..665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(649..665)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(662..684)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    673..689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(697..703,704..721,722..727))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    742..763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(880..897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaattgggc cctctagatg catgctcgag cggccgccag tgtgatggat atctgcagaa
       61 ttcgccctta tatagatgcc gtcctagcgc gtctcctgac cccagagcag gtcgtggcca
      121 ttgcctcgaa tggagggggc aaacaggcgt tggaaaccgt acaacgattg ctgccggtgc
      181 tgtgccaagc gcacggactc accccagagc aggtcgtggc gatcgcaagc cacgacggag
      241 gaaagcaagc cttggaaaca gtacagaggc tgttgcctgt gctgtgccaa gcgcacggcc
      301 tcaccccaga gcaggtcgtg gccattgcct cgaatggagg gggcaaacag gcgttggaaa
      361 ccgtacaacg attgctgccg gtgctgtgcc aagcgcacgg attaacccca gagcaggtcg
      421 tggcgatcgc aagccacgac ggaggaaagc aagccttgga aacagtacag aggctgttgc
      481 ctgtgctgtg ccaagcgcac ggcttaagag acgtgggcac aggaaagata cttaagggcg
      541 aattccagca cactggcggc cgttactagt ggatccgagc tcggtaccaa gcttgatgca
      601 tagcttgagt attctatagt gtcacctaaa tagcttggcg taatcatggt catagctgtt
      661 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
      721 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
      781 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
      841 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
      901 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaaggcggta atacggttat
      961 ccacagaatc agggg
//
