LOCUS       sequence-61964-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-TTGT
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(618..636)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(619..636)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(654..670)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(654..670)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(667..689)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    678..694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(702..708,709..726,727..732))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    747..768
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(885..902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tagggcgaat tgggccctct agatgcatgc tcgagcggcc gccagtgtga tggatatctg
       61 cagaattcgc ccttaagtat ctttcctgtg cccacgtctc ttaagccgtg cgcttggcac
      121 agcaccggca gcaatcgttg tacggtttcc aacgcctgtt tgccccctcc attcgaggca
      181 atggccacga cctgctctgg ggttaatccg tgcgcttggc acagcactgg aaggagcctc
      241 tgcaccgttt ccaaagcctg ttttccgccg ttattgctcg cgattgccac gacctgctct
      301 ggggtgaggc cgtgcgcttg gcacagcacc ggcagcaatc gttgtacggt ttccaacgcc
      361 tgtttgcccc ctccattcga ggcaatggcc acgacctgct ctggggtgag tccgtgcgct
      421 tggcacagca ccggcagcaa tcgttgtacg gtttccaacg cctgtttgcc ccctccattc
      481 gaggcaatgg ccacgacctg ctctggggtc aggagacgcg ctaggacggc atctatataa
      541 gggcgaattc cagcacactg gcggccgtta ctagtggatc cgagctcggt accaagcttg
      601 atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag
      661 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      721 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      781 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      841 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      901 ctgcgctcgg tcgttcggct gcggcgaagc ggtatcagct cactcaaa
//
