LOCUS       sequence-61968-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-TCGC
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(602..620)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(603..620)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(638..654)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(638..654)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(651..673)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    662..678
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(686..692,693..710,711..716))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    731..752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(869..886)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgaattgg gcccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcgccc ttgccgtcct agcgcgtctc ctgaccccag agcaggtcgt ggccattgcc
      121 tcgaatggag ggggcaaaca ggcgttggaa accgtacaac gattgctgcc ggtgctgtgc
      181 caagcgcacg gactcacccc agagcaggtc gtggcgatcg caagccacga cggaggaaag
      241 caagccttgg aaacagtaca gaggctgttg cctgtgctgt gccaagcgca cggcctcacc
      301 ccagagcagg tcgtggcaat cgcgagcaat aacggcggaa aacaggcttt ggaaacggtg
      361 cagaggctcc ttccagtgct gtgccaagcg cacggattaa ccccagagca ggtcgtggcg
      421 atcgcaagcc acgacggagg aaagcaagcc ttggaaacag tacagaggct gttgcctgtg
      481 ctgtgccaag cgcacggctt aagagacgtg ggcacaggaa agaagggcga attccagcac
      541 actggcggcc gttactagtg gatccgagct cggtaccaag cttgatgcat agcttgagta
      601 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      661 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      721 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      781 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      841 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaa
//
