LOCUS       sequence-61991-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-TTAT
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(609..625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(609..625)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(622..644)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    633..649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(657..663,664..681,682..687))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    702..723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(840..857)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgaattgg gccctctaga tgcatgctcg agcggccgcc agtgtgatgg atatctgcag
       61 aattcgccct tatatagatg ccgtcctagc gcgtctcctg accccagagc aggtcgtggc
      121 cattgcctcg aatggagggg gcaaacaggc gttggaaacc gtacaacgat tgctgccggt
      181 gctgtgccaa gcgcacggac tcaccccaga gcaggtcgtg gccattgcct cgaatggagg
      241 gggcaaacag gcgttggaaa ccgtacaacg attgctgccg gtgctgtgcc aagcgcacgg
      301 cctcacccca gagcaggtcg tggcaatcgc ctccaacatt ggcgggaaac aggcactcga
      361 gactgtccag cgcctgcttc ccgtgctgtg ccaagcgcac ggattaaccc cagagcaggt
      421 cgtggccatt gcctcgaatg gagggggcaa acaggcgttg gaaaccgtac aacgattgct
      481 gccggtgctg tgccaagcgc acggcttaag agacgtgggc acaggaaaga tacttaaggg
      541 cgaattccag cacactggcg gccgttacta gtggatccga gctcggtacc aagcttggcg
      601 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      661 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      721 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      781 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      841 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      901 aaggcggtaa tacggttatc cacagaatca ggggataacg ca
//
