LOCUS       sequence-61994-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pZC05 (pFA6a-TEV-6xGly-PrtA-KanMX)
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..60
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             121..294
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             295..468
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     terminator      527..714
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(582..606)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 tcgtacgctg caggtcgacg gatccccggg ttaattaacg agaatttata cttccaaggt
       61 tctggtggag gcggtggagg ctctagaaaa accgcggctc ttgcgcaaca cgatgaagcc
      121 gtggacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt acatttacct
      181 aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga cccaagccaa
      241 agcgctaacc ttttagcaga agctaaaaag ctaaatgatg ctcaggcgcc gaaagtagac
      301 aacaaattca acaaagaaca acaaaacgcg ttctatgaga tcttacattt acctaactta
      361 aacgaagaac aacgaaacgc cttcatccaa agtttaaaag atgacccaag ccaaagcgct
      421 aaccttttag cagaagctaa aaagctaaat ggtgctcagg cgccgaaagt agacgcgaat
      481 tccgcgggga agtcaacctg actcgaggcg cgccacttct aaataagcga atttcttatg
      541 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
      601 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
      661 gttgctttct caggtatagt atgaggtcgc tcttattgac cacacctcta ccggcagatc
      721 cgctagggat aacagggtaa tatagatctg tttagcttgc ctcgtccccg ccgggtcacc
      781 cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg cgcagctcag
      841 gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta taatcatttg
      901 catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacgg
//
