LOCUS       sequence-61999-e        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-TTCC
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(617..635)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(618..635)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(653..669)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(653..669)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(666..688)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    677..693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(701..707,708..725,726..731))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    746..767
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(884..901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgggcgaat tgggccctct gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattcgcc cttatataga tgccgtccta gcgcgtctcc tgaccccaga gcaggtcgtg
      121 gccattgcct cgaatggagg gggcaaacag gcgttggaaa ccgtacaacg attgctgccg
      181 gtgctgtgcc aagcgcacgg actcacccca gagcaggtcg tggccattgc ctcgaatgga
      241 gggggcaaac aggcgttgga aaccgtacaa cgattgctgc cggtgctgtg ccaagcgcac
      301 ggcctcaccc cagagcaggt cgtggcgatc gcaagccacg acggaggaaa gcaagccttg
      361 gaaacagtac agaggctgtt gcctgtgctg tgccaagcgc acggattaac cccagagcag
      421 gtcgtggcga tcgcaagcca cgacggagga aagcaagcct tggaaacagt acagaggctg
      481 ttgcctgtgc tgtgccaagc gcacggctta agagacgtgg gcacaggaaa gatacttaag
      541 ggcgaattcc agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga
      601 tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc
      661 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      721 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      781 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      841 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      901 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      961 tatccacaga atcaggggat anccagagaa anancatgtg agcaaaaggc ca
//
