LOCUS       sequence-62-expo        4263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD52_102
FEATURES             Location/Qualifiers
     source          1..4263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1837..1859)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1959..1978
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2056..2074)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2142..2246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2247..2315,2316..3107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2465..2484)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3278..3866
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3767..3786
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4020..4037
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4154..4175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4190..4195,4196..4213,4214..4220)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4228..4244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4233..4255
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4252..4263,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4252..4263,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagaaat gtaaacctgt
       61 catttctgtg tatttcagca ccgcgaagag caccataaac agctacaaca agagcacgaa
      121 cgtcggctcc aacagaaaaa gctgcaaaac gctggtactc accacatgct ctgataattg
      181 ccatttcctt cgatttctga ctttcaattg tgatatgata acctcaaaat ctccattcaa
      241 aatgaaaact cttaaattta agagctttca acaccccgca ctttcccacc tcatcgtttt
      301 tcccttctat cttatcaatt ttttgtattt tgtgtcagtt tgctatatcc tcatcacctt
      361 ttttaccgtt taatctctct atagtattgt tcgggtgttt caagatgaaa cgttttgtgt
      421 gtagcatttt tgaaacacag cgcgaaaaat gcagaaacta tcatccacaa tgcattaagt
      481 gcactcattg tgatgactcc taaccccctc gccccaccag ttgtctcttc acaaattcat
      541 ggcacaattc aaaaatggct ttttgtgttg gatcttgatc tcgtctcttt ccttcttctt
      601 tcgtttctct cggaccactc attattcgaa tgccatgcat ttgttgatga tgcgcagcga
      661 tggtcgcaca cactacaaca gatatgatgg tggctgtgcg tggtgcagag ctcatgaata
      721 agtgatgggg caccgagatg tgactcctcc cattattcgt cttgagtcgc atcttttgtc
      781 ttggtccgtt gtcagtcgag cctcaaggca gccgttccgc cagtgatcgc ctgctaagaa
      841 gttgtaggtg tagaggaaga aacgtcgggt ccaaatttca agcaacatcc gcgagatcgt
      901 gaatcccggg gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat
      961 agaacatttt caggaggacc cttggctagc gtcgacggta ccatggtatt gatatctgag
     1021 ctccgcatcg gccgctgtca tcagatcgcc atctcgcgcc cgtgcctctg acttctaagt
     1081 ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt
     1141 gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct
     1201 aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa
     1261 ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt
     1321 acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca
     1381 cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc
     1441 acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt
     1501 tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg
     1561 gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt
     1621 attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag
     1681 gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg
     1741 acagaataca ttcccaatat accaaacata actgtttcct actagtcggc cgtacgggcc
     1801 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     1861 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     1921 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     1981 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     2041 caggcggcct taagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2101 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2161 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2221 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2281 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2341 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2401 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2461 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2521 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2581 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2641 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2701 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2761 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2821 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2881 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2941 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3001 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3061 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3121 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3181 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3241 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3301 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3361 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3421 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3481 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3541 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3601 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3661 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3721 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3781 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3841 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3901 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3961 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4021 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4081 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4141 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     4201 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     4261 gct
//
