LOCUS       sequence-62016-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..54
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             82..105
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             142..165
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      194..381
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(249..273)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        456..799
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(867..886)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacgagaatt tatacttcca aggttctggt
       61 ggaggcggtg gaggctctag atggtctcac ccacagttcg agaagggtgg aggctctggc
      121 ggtggatccg gaggcggttc atggtctcac ccacagttcg agaagtgact cgaggcgcgc
      181 cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
      241 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt
      301 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagtatg aggtcgctct
      361 tattgaccac acctctaccg gcagatccgc tagggataac agggtaatat agatctgttt
      421 agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc
      481 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc
      541 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa
      601 gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg
      661 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac
      721 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca
      781 catccgaaca taaacaacca tgggtaagga aaagactcac gtttcgaggc cgcgattaaa
      841 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
      901 aggtgcgaca atctatcgat tgtatgggaa gccc
//
