LOCUS       sequence-62062-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTF259 (pFA6a-TEV-6xGly-KanMX)
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..60
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      119..306
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(174..198)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        381..724
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(792..811)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tcgtacgctg caggtcgacg gatccccggg ttaattaacg agaatttata cttccaaggt
       61 tctggtggag gcggtggagg ctctagaggt accctcgagg cgcgccactt ctaaataagc
      121 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata
      181 caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt
      241 cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc gctcttattg accacacctc
      301 taccggcaga tccgctaggg ataacagggt aatatagatc tgtttagctt gcctcgtccc
      361 cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg
      421 tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg
      481 tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct
      541 cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc
      601 acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc
      661 atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac
      721 aaccatgggt aaggaaaaga ctcacgtttc gaggccgcga ttaaattcca acatggatgc
      781 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
      841 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
      901 tgccaatgat gt
//
