LOCUS       sequence-62072-e        4900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized EGFP
            with CaUra3 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yoEGFP-CaURA3
FEATURES             Location/Qualifiers
     source          1..4900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..55)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     155..174
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        255..273
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     255..272
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(343..345,346..1056)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     terminator      1080..1267
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1135..1159)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1342..1685
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2504..2701
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2808..2827)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2809..2827)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2914..2931)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3085..3673)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3165..3184)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3844..4635,4636..4704))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4467..4486
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4705..4809)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4877..4895
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
       61 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
      121 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
      181 gtgcaccata tggacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt
      241 atcatacaca tacgatttag gtgacactat agaacgcggc cgccagctga agcttcgtac
      301 gctgcaggtc gacggatcgg tgacggtgct ggtttaatta acatgtcaaa aggcgaggaa
      361 ctattcacag gtgtcgtacc aatactggta gaattggatg gcgacgtaaa cgggcataag
      421 ttttctgtga gtggtgaggg tgaaggggac gcaacttatg gcaaacttac acttaagttc
      481 atctgcacca cagggaagct gcctgtccca tggccaactc tagttacaac tctcacttac
      541 ggagtacaat gtttttctcg atacccagac cacatgaaac aacatgactt tttcaaatct
      601 gccatgcctg agggatatgt gcaagagaga actattttct ttaaagatga tggtaattac
      661 aaaactcgtg ctgaagtgaa gtttgaagga gatacacttg ttaatagaat tgagttaaag
      721 ggtattgact ttaaggaaga tggtaatatc ttaggtcaca aattggaata caactacaat
      781 tcacataacg tttacatcat ggcagataaa cagaaaaacg gcataaaagt taatttcaaa
      841 attagacata atatcgaaga tggttccgtt caactcgctg atcattatca acaaaataca
      901 ccaatcggag atggacctgt tctattgcca gacaaccatt acttatctac acagagcgcc
      961 ttgtcaaaag atcctaatga aaagagagat cacatggttt tgttagaatt tgtcaccgct
     1021 gcgggtataa cgttgggcat ggatgaatta tacaagtaag gcgcgccact tctaaataag
     1081 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
     1141 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt
     1201 tcctgtaggt caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct
     1261 ctaccggcag atccgctagg gataacaggg taatatagat ctgtttagct tgcctcgtcc
     1321 ccgccgggtc acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac
     1381 gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat
     1441 gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc
     1501 tcctcgctgc agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc
     1561 cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt
     1621 catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa
     1681 caaccatgac agtcaacact aagacctata gtgagagagc agaaactcat gcctcaccag
     1741 tagcacagcg attatttcga ttaatggaac tgaagaaaac caatttatgt gcatcaattg
     1801 acgttgatac cactaaggaa ttccttgaat taattgataa attaggtcct tatgtatgct
     1861 taatcaagac tcatattgat ataatcaatg atttttccta tgaatccact attgaaccat
     1921 tattagaact ttcacgtaaa catcaattta tgatttttga agatagaaaa tttgctgata
     1981 ttggtaatac cgtaaagaaa caatatattg gtggagttta taaaattagt agttgggcag
     2041 atattaccaa tgctcatggt gtcactggga atggagtggt tgaaggatta aaacagggag
     2101 ctaaagaaac caccaccaac caagagccaa gagggttatt gatgttagct gaattatcat
     2161 cagtgggatc attagcatat ggagaatatt ctcaaaaaac tgttgaaatt gctaaatccg
     2221 ataaggaatt tgttattgga tttattgccc aacgtgatat gggtggccaa gaagaaggat
     2281 ttgattggct tattatgaca cctggagttg gattagatga taaaggtgat ggattaggac
     2341 aacaatatag aactgttgat gaagttgtta gcactggaac tgatattatc attgttggta
     2401 gaggattgtt tggtaaagga agagatccag atattgaagg taaaaggtat agaaatgctg
     2461 gttggaatgc ttatttgaaa aagactggcc aattataatc agtactgaca ataaaaagat
     2521 tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta
     2581 atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa
     2641 gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact
     2701 gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa acgagctcga attcatcgat
     2761 gatatcagat ccactagtgg cctatgcggc cgcggatctg ccggtctccc tatagtgagt
     2821 cgtattaatt tcgataagcc aggttaacct gcattaatga atcggccaac gcgcggggag
     2881 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2941 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3001 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3061 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3121 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3181 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3241 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     3301 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3361 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3421 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3481 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3541 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3601 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3661 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3721 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3781 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3841 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3901 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3961 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4021 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4081 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4141 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4201 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4261 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4321 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4381 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4441 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4501 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4561 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4621 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4681 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4741 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4801 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     4861 gacattaacc tataaaaata ggcgtatcac gaggcccttt
//
