LOCUS       sequence-62075-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B
  TITLE     An improved short-lived fluorescent protein transcriptional reporter
            for Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 
            21.
  PUBMED    23172645
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2932"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "519-30"
COMMENT     Sequence Label: pNC1124
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(454..784)
                     /gene="S. cerevisiae GAL10"
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    667..784
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 acagctatga catgattacg aattcgagct ctggagaata tactaagggt actgttgaca
       61 ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa
      121 gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
      181 acgcattggg tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta
      241 ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt
      301 acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg
      361 tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
      421 agttattacc cggtaccccc ctcgaggaat tttcaaaaat tcttactttt tttttggatg
      481 gacgcaaaga agtttaataa tcatattaca tggcattacc accatataca tatccatata
      541 catatccata tctaatctta cttatatgtt gtggaaatgt aaagagcccc attatcttag
      601 cctaaaaaaa ccttctcttt ggaactttca gtaatacgct taactgctca ttgctatatt
      661 gaagtacgga ttagaagccg ccgagcgggt gacagccctc cgaaggaaga ctctcctccg
      721 tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc
      781 tccgaacaat aaagattcta caatactacc ttttatggtt atgaag
//
