LOCUS       sequence-62081-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pZC14 (pFA6a-PSc-6xGly-V5-HphMX)
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..63
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             88..129
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      158..345
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(213..237)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        420..763
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 tcgtacgctg caggtcgacg gatccccggg ttaattaact tggaagtatt gttccaaggt
       61 ccaggtggag gcggtggagg ctctagaggt aagccaattc caaacccatt gttaggtttg
      121 gattctactt gactcgaggc gcgccacttc taaataagcg aatttcttat gatttatgat
      181 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
      241 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
      301 tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat ccgctaggga
      361 taacagggta atatagatct gtttagcttg cctcgtcccc gccgggtcac ccggccagcg
      421 acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat
      481 gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac
      541 attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcgg acctgcgagc
      601 agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa
      661 atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct
      721 tgctaggata cagttctcac atcacatccg aacataaaca accatgggta aaaagcctga
      781 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
      841 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgta
//
