LOCUS       sequence-62092-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-GATT
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        14..32
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     14..31
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(645..664)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(646..664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(671..688)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(671..687)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(680..702)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc aagcttggta
       61 ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattcgccct taagtatctt
      121 tcctgtgccc acgtctctta agccgtgcgc ttggcacagc accggcagca atcgttgtac
      181 ggtttccaac gcctgtttgc cccctccatt cgaggcaatg gccacgacct gctctggggt
      241 taatccgtgc gcttggcaca gcaccggcag caatcgttgt acggtttcca acgcctgttt
      301 gccccctcca ttcgaggcaa tggccacgac ctgctctggg gtgaggccgt gcgcttggca
      361 cagcacggga agcaggcgct ggacagtctc gagtgcctgt ttcccgccaa tgttggaggc
      421 gattgccacg acctgctctg gggtgagtcc gtgcgcttgg cacagcactg gaaggagcct
      481 ctgcaccgtt tccaaagcct gttttccgcc gttattgctc gcgattgcca cgacctgctc
      541 tggggtcagg agacgcgcta ggacggcatc tatataaggg cgaattctgc agatatccat
      601 cacactggcg gccgctcgag catgcatcta gagggcccaa ttcgccctat agtgagtcgt
      661 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      721 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      781 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
      841 cggcgcatta a
//
