LOCUS       sequence-62097-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: I-GAAA
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        14..32
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     14..31
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(631..650)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(632..650)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(657..674)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(657..673)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(666..688)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc aagcttggta
       61 ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattcgccct tgccgtccta
      121 gcgcgtctcc tgaccccaga gcaggtcgtg gcaatcgcga gcaataacgg cggaaaacag
      181 gctttggaaa cggtgcagag gctccttcca gtgctgtgac aagcgcacgg actcacccca
      241 gagcaggtcg tggcaatcgc ctccaacatt ggcgggaaac aggcactcga gactgtccag
      301 cgcctgcttc ccgtgctgtg ccaagcgcac ggcctcaccc cagagcaggt cgtggcaatc
      361 gcctccaaca ttggcgggaa acaggcactc gagactgtcc agcgcctgct tcccgtgctg
      421 tgccaagcgc acggattaac cccagagcag gtcgtggcaa tcgcctccaa cattggcggg
      481 aaacaggcac tcgagactgt ccagcgcctg cttcccgtgc tgtgccaagc gcacggctta
      541 agagacgtgg gcacaggaaa gaagggcgaa ttctgcagat atccatcaca ctggcggccg
      601 ctcgagcatg catctagagg gcccaattcg ccctatagtg agtcgtatta caattcactg
      661 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      721 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      781 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
      841 cggcgggtgt ggtggttacg cgcag
//
