LOCUS       sequence-62114-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: IV-GTG
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(468..484)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(468..484)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(481..503)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    492..508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(516..522,523..540,541..546))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    561..582
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(699..716)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
       61 ctgcagaatt cgcccttata tctttcctgt gcccacgtct ctctccagtg cgggtcttcc
      121 gcccttattg ctcgcgattg ccacgacctg ctctggggtt aatccgtgcg cttggcacag
      181 caccggcagc aatcgttgta cggtttccaa cgcctgtttg ccccctccat tcgaggcaat
      241 ggccacgacc tgctctgggg tgaggccgtg cgcttggcac agcactggaa ggagcctctg
      301 caccgtttcc aaagcctgtt ttccgccgtt attgctcgcg attgccacga cctgctctgg
      361 ggtgagtcga gacgcgctag gacggcatct atataagggc gaattccagc acactggcgg
      421 ccgttactag tggatccgag ctcggtacca agcttggcgt aatcatggtc atagctgttt
      481 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      541 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      601 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      661 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
      721 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      781 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      841 aaccgtaaaa agg
//
