LOCUS       sequence-62123-e        4882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized 
            mKate2 with CaUra3 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yomKate2-CaURA3
FEATURES             Location/Qualifiers
     source          1..4882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     104..123
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        204..222
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     204..221
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1011..1198
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1066..1090)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1273..1616
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2435..2632
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2739..2758)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2740..2758)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2845..2862)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3016..3604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(3775..4566,4567..4635))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4398..4417
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4636..4740)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4808..4826
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(join(4864..4882,1..4))
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
       61 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
      121 gtactgagag tgcaccatat ggacatattg tcgttagaac gcggctacaa ttaatacata
      181 accttatgta tcatacacat acgatttagg tgacactata gaacgcggcc gccagctgaa
      241 gcttcgtacg ctgcaggtcg acggatcggt gacggtgctg gtttaattaa catggtttct
      301 gaactcatca aggaaaacat gcacatgaaa ctttacatgg aaggtactgt gaacaatcat
      361 cattttaagt gtacatccga gggtgaaggc aaaccttacg aaggaactca aactatgaga
      421 attaaagctg tagaaggtgg accattacct tttgcatttg atatcttggc aacatcattc
      481 atgtatggga gcaagacatt cataaaccat actcaaggta taccagactt tttcaaacag
      541 agttttccag agggttttac atgggaaaga gtaacaacgt acgaggatgg aggtgtattg
      601 acagccactc aagacacatc acttcaagat gggtgtttaa tctacaatgt caagattaga
      661 ggcgtcaatt tcccttctaa tggtccagtt atgcagaaaa agacattagg ctgggaagcg
      721 tcaaccgaaa ccctgtaccc tgctgatggt ggcctagaag gcagagctga catggccctt
      781 aaactggttg gtggagggca tctaatctgc aatttgaaaa ccacttatcg ttctaaaaag
      841 ccagccaaaa acctaaagat gccaggtgtt tactacgtcg accgaagatt agaaaggatt
      901 aaagaggctg ataaagagac ttatgttgaa caacacgaag tggcagtggc tagatactgt
      961 gatttgccat ctaagttggg acacagataa ggcgcgccac ttctaaataa gcgaatttct
     1021 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     1081 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     1141 tcaggttgct ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca
     1201 gatccgctag ggataacagg gtaatataga tctgtttagc ttgcctcgtc cccgccgggt
     1261 cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc
     1321 tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca
     1381 tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg
     1441 cagacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg
     1501 cccctgtaga gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt
     1561 ccttttaaaa tcttgctagg atacagttct cacatcacat ccgaacataa acaaccatga
     1621 cagtcaacac taagacctat agtgagagag cagaaactca tgcctcacca gtagcacagc
     1681 gattatttcg attaatggaa ctgaagaaaa ccaatttatg tgcatcaatt gacgttgata
     1741 ccactaagga attccttgaa ttaattgata aattaggtcc ttatgtatgc ttaatcaaga
     1801 ctcatattga tataatcaat gatttttcct atgaatccac tattgaacca ttattagaac
     1861 tttcacgtaa acatcaattt atgatttttg aagatagaaa atttgctgat attggtaata
     1921 ccgtaaagaa acaatatatt ggtggagttt ataaaattag tagttgggca gatattacca
     1981 atgctcatgg tgtcactggg aatggagtgg ttgaaggatt aaaacaggga gctaaagaaa
     2041 ccaccaccaa ccaagagcca agagggttat tgatgttagc tgaattatca tcagtgggat
     2101 cattagcata tggagaatat tctcaaaaaa ctgttgaaat tgctaaatcc gataaggaat
     2161 ttgttattgg atttattgcc caacgtgata tgggtggcca agaagaagga tttgattggc
     2221 ttattatgac acctggagtt ggattagatg ataaaggtga tggattagga caacaatata
     2281 gaactgttga tgaagttgtt agcactggaa ctgatattat cattgttggt agaggattgt
     2341 ttggtaaagg aagagatcca gatattgaag gtaaaaggta tagaaatgct ggttggaatg
     2401 cttatttgaa aaagactggc caattataat cagtactgac aataaaaaga ttcttgtttt
     2461 caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt
     2521 tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc
     2581 gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat
     2641 tcgatactaa cgccgccatc cagtgtcgaa aacgagctcg aattcatcga tgatatcaga
     2701 tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag tcgtattaat
     2761 ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2821 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2881 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2941 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3001 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3061 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3181 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     3241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3421 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3601 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3661 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3721 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3781 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3841 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3901 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3961 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4021 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4081 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4141 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4201 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4261 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4321 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4381 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4441 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4501 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4561 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4621 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4681 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4741 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     4801 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     4861 aaacctctga cacatgcagc tc
//
