LOCUS       sequence-62125-e        4894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized 
            mApple with CaUra3 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yomApple-CaURA3
FEATURES             Location/Qualifiers
     source          1..4894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     75..94
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        175..193
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     175..192
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      994..1181
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1049..1073)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1256..1599
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2418..2615
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2722..2741)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2723..2741)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2828..2845)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2999..3587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3079..3098)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3758..4549,4550..4618))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4381..4400
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4619..4723)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4791..4809
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4847..4869)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
       61 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt
      121 gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
      181 gtgacactat agaacgcggc cgccagctga agcttcgtac gctgcaggtc gacggatcgg
      241 tgacggtgct ggtttaatta acatggtatc taaaggtgag gaaaacaata tggctatcat
      301 taaagagttt atgcgtttca aggttcacat ggaaggttct gttaatggtc atgagttcga
      361 aatagaaggt gagggcgaag ggcgaccata cgaagcattt caaactgcta aactcaaggt
      421 aactaaaggc ggtcctcttc catttgcctg ggatatattg tcacctcagt tcatgtatgg
      481 ttcaaaagtt tacattaaac atccagcgga tatccctgac tactttaaac tgtctttccc
      541 tgaaggtttt agatgggaaa gagttatgaa cttcgaagat ggtggaatta ttcacgtcaa
      601 tcaagattca agtttacagg atggcgtctt tatctacaag gtgaagttga gaggaacaaa
      661 ttttccatcc gatgggccag ttatgcaaaa gaaaactatg ggctgggaag cctctgaaga
      721 gagaatgtac ccagaggacg gagccttaaa aagtgaaatc aaaaagagat tgaagttgaa
      781 ggatggtggg cactatgcag cagaagtgaa aacgacatat aaggctaaaa agcctgtcca
      841 actaccagga gcttatattg tggacatcaa acttgacata gtaagccata acgaagatta
      901 caccatcgtt gaacaatacg agagagctga aggtaggcat tccacaggag gcatggacga
      961 actatacaaa taaggcgcgc cacttctaaa taagcgaatt tcttatgatt tatgattttt
     1021 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
     1081 ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
     1141 gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc tagggataac
     1201 agggtaatat agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat
     1261 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
     1321 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
     1381 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg
     1441 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
     1501 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
     1561 aggatacagt tctcacatca catccgaaca taaacaacca tgacagtcaa cactaagacc
     1621 tatagtgaga gagcagaaac tcatgcctca ccagtagcac agcgattatt tcgattaatg
     1681 gaactgaaga aaaccaattt atgtgcatca attgacgttg ataccactaa ggaattcctt
     1741 gaattaattg ataaattagg tccttatgta tgcttaatca agactcatat tgatataatc
     1801 aatgattttt cctatgaatc cactattgaa ccattattag aactttcacg taaacatcaa
     1861 tttatgattt ttgaagatag aaaatttgct gatattggta ataccgtaaa gaaacaatat
     1921 attggtggag tttataaaat tagtagttgg gcagatatta ccaatgctca tggtgtcact
     1981 gggaatggag tggttgaagg attaaaacag ggagctaaag aaaccaccac caaccaagag
     2041 ccaagagggt tattgatgtt agctgaatta tcatcagtgg gatcattagc atatggagaa
     2101 tattctcaaa aaactgttga aattgctaaa tccgataagg aatttgttat tggatttatt
     2161 gcccaacgtg atatgggtgg ccaagaagaa ggatttgatt ggcttattat gacacctgga
     2221 gttggattag atgataaagg tgatggatta ggacaacaat atagaactgt tgatgaagtt
     2281 gttagcactg gaactgatat tatcattgtt ggtagaggat tgtttggtaa aggaagagat
     2341 ccagatattg aaggtaaaag gtatagaaat gctggttgga atgcttattt gaaaaagact
     2401 ggccaattat aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg
     2461 tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt
     2521 tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat
     2581 gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc
     2641 atccagtgtc gaaaacgagc tcgaattcat cgatgatatc agatccacta gtggcctatg
     2701 cggccgcgga tctgccggtc tccctatagt gagtcgtatt aatttcgata agccaggtta
     2761 acctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2821 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2881 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2941 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3001 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3061 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3121 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3181 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3241 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3301 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3361 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3421 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3481 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3541 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3601 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3661 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3721 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3781 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3841 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3901 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3961 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4021 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4081 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4141 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4201 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4261 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4321 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4381 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4441 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4501 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4561 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4621 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4681 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4741 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4801 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     4861 agctcccgga gacggtcaca gcttgtctgt aagc
//
