LOCUS       sequence-62136-e        4900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized 
            mEmerald with CaUra3 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yomEmerald-CaURA3
FEATURES             Location/Qualifiers
     source          1..4900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(52..74)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     174..193
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        274..292
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     274..291
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1099..1286
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1154..1178)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1361..1704
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2523..2720
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2827..2846)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2828..2846)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2933..2950)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3104..3692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3184..3203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3863..4654,4655..4723))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4486..4505
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4724..4828)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(4896..4900,1..14)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
       61 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
      121 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
      181 agagcagatt gtactgagag tgcaccatat ggacatattg tcgttagaac gcggctacaa
      241 ttaatacata accttatgta tcatacacat acgatttagg tgacactata gaacgcggcc
      301 gccagctgaa gcttcgtacg ctgcaggtcg acggatcggt gacggtgctg gtttaattaa
      361 catgtcaaaa ggcgaggaac tattcacagg tgtcgtacca atactggtag aattggatgg
      421 cgacgtaaac gggcataagt tttctgtgag tggtgagggt gaaggggacg caacttatgg
      481 caaacttaca cttaagttca tctgcaccac agggaagctg cctgtcccat ggccaactct
      541 agttacaact ctcacttacg gagtacaatg ttttgcacga tacccagacc acatgaaaca
      601 acatgacttt ttcaaatctg ccatgcctga gggatatgtg caagagagaa ctattttctt
      661 taaagatgat ggtaattaca aaactcgtgc tgaagtgaag tttgaaggag atacacttgt
      721 taatagaatt gagttaaagg gtattgactt taaggaagat ggtaatatct taggtcacaa
      781 attggaatac aactacaatt cacataaggt ttacatcaca gcagataaac agaaaaacgg
      841 cataaaagtt aatttcaaaa ccagacataa tatcgaagat ggttccgttc aactcgctga
      901 tcattatcaa caaaatacac caatcggaga tggacctgtt ctattgccag acaaccatta
      961 cttatctaca cagagcgcct tgtcaaaaga tcctaatgaa aagagagatc acatggtttt
     1021 gttagaattt gtcaccgctg cgggtataac gttgggcatg gatgaattat acaagtaagg
     1081 cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
     1141 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     1201 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc
     1261 gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc
     1321 tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac
     1381 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat
     1441 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc
     1501 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac
     1561 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt
     1621 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca
     1681 catcacatcc gaacataaac aaccatgaca gtcaacacta agacctatag tgagagagca
     1741 gaaactcatg cctcaccagt agcacagcga ttatttcgat taatggaact gaagaaaacc
     1801 aatttatgtg catcaattga cgttgatacc actaaggaat tccttgaatt aattgataaa
     1861 ttaggtcctt atgtatgctt aatcaagact catattgata taatcaatga tttttcctat
     1921 gaatccacta ttgaaccatt attagaactt tcacgtaaac atcaatttat gatttttgaa
     1981 gatagaaaat ttgctgatat tggtaatacc gtaaagaaac aatatattgg tggagtttat
     2041 aaaattagta gttgggcaga tattaccaat gctcatggtg tcactgggaa tggagtggtt
     2101 gaaggattaa aacagggagc taaagaaacc accaccaacc aagagccaag agggttattg
     2161 atgttagctg aattatcatc agtgggatca ttagcatatg gagaatattc tcaaaaaact
     2221 gttgaaattg ctaaatccga taaggaattt gttattggat ttattgccca acgtgatatg
     2281 ggtggccaag aagaaggatt tgattggctt attatgacac ctggagttgg attagatgat
     2341 aaaggtgatg gattaggaca acaatataga actgttgatg aagttgttag cactggaact
     2401 gatattatca ttgttggtag aggattgttt ggtaaaggaa gagatccaga tattgaaggt
     2461 aaaaggtata gaaatgctgg ttggaatgct tatttgaaaa agactggcca attataatca
     2521 gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata
     2581 ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca
     2641 tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt
     2701 gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca gtgtcgaaaa
     2761 cgagctcgaa ttcatcgatg atatcagatc cactagtggc ctatgcggcc gcggatctgc
     2821 cggtctccct atagtgagtc gtattaattt cgataagcca ggttaacctg cattaatgaa
     2881 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2941 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3001 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3061 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3121 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3181 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3241 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     3301 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3361 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3421 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3481 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3541 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3601 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3661 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3721 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3781 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3841 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3901 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3961 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4021 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4081 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4141 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4201 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4261 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4321 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4381 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4441 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4501 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4561 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4621 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4681 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4741 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4801 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     4861 aagaaaccat tattatcatg acattaacct ataaaaatag
//
