LOCUS       sequence-62150-e        4888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized 
            mCherry with Kan selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yomCherry-Kan
FEATURES             Location/Qualifiers
     source          1..4888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..60
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        141..159
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     141..158
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      960..1147
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1015..1039)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            1222..2578
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1222..1565
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1566..2375
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1633..1652)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2381..2578
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2682..2701)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2683..2701)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2788..2805)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2959..3547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3039..3058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3718..4509,4510..4578))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4341..4360
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4579..4683)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4751..4769
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4807..4829)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
       61 ctgagagtgc accatatgga catattgtcg ttagaacgcg gctacaatta atacataacc
      121 ttatgtatca tacacatacg atttaggtga cactatagaa cgcggccgcc agctgaagct
      181 tcgtacgctg caggtcgacg gatcggtgac ggtgctggtt taattaacat ggttagcaaa
      241 ggcgaggaag ataacatggc tataatcaaa gagtttatga gattcaaagt acacatggag
      301 ggttcagtga atggtcatga atttgaaatt gaaggcgaag gcgagggcag accttacgaa
      361 ggaactcaaa cagcaaaact taaggtaaca aaaggtggtc ctctgccatt cgcctgggac
      421 attctcagtc cacaattcat gtacggttct aaagcgtacg tcaaacatcc agcagacatt
      481 ccagattact tgaaattgtc ttttccagaa ggctttaagt gggaaagagt tatgaacttc
      541 gaggatggag gggttgtgac cgttacgcaa gattcctctt tacaagatgg tgagtttatc
      601 tacaaggtca aattaagggg gactaatttt ccttcagacg ggccagtcat gcagaaaaag
      661 actatgggat gggaagcctc ttcagagaga atgtatcctg aagatggcgc tctaaaagga
      721 gaaatcaagc aaagattgaa gttaaaggac ggaggtcatt atgatgcaga agtaaaaaca
      781 acctataaag ctaaaaagcc agttcaactt cctggtgcct acaatgttaa catcaagcta
      841 gacattacat cccataatga agattacact atagtggaac agtatgaacg tgctgaaggt
      901 agacacagta caggtggtat ggatgaactg tacaagtaag gcgcgccact tctaaataag
      961 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
     1021 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt
     1081 tcctgtaggt caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct
     1141 ctaccggcag atccgctagg gataacaggg taatatagat ctgtttagct tgcctcgtcc
     1201 ccgccgggtc acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac
     1261 gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat
     1321 gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc
     1381 tcctcgctgc agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc
     1441 cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt
     1501 catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa
     1561 caaccatggg taaggaaaag actcacgttt cgaggccgcg attaaattcc aacatggatg
     1621 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     1681 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     1741 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     1801 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     1861 tccccggcaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     1921 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     1981 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     2041 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     2101 aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     2161 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     2221 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     2281 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     2341 tgcagtttca tttgatgctc gatgagtttt tctaatcagt actgacaata aaaagattct
     2401 tgttttcaag aacttgtcat ttgtatagtt tttttatatt gtagttgttc tattttaatc
     2461 aaatgttagc gtgatttata ttttttttcg cctcgacatc atctgcccag atgcgaagtt
     2521 aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga atgctggtcg ctatactgct
     2581 gtcgattcga tactaacgcc gccatccagt ttaaacgagc tcgaattcat cgatgatatc
     2641 agatccacta gtggcctatg cggccgcgga tctgccggtc tccctatagt gagtcgtatt
     2701 aatttcgata agccaggtta acctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     2761 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2821 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2881 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2941 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3001 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3061 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3121 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     3181 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3301 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3361 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     3421 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3481 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3541 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3601 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3661 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3721 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3781 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3841 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3901 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3961 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4021 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4081 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4141 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4201 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4261 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4321 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4381 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4441 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4501 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4561 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4621 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4681 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     4741 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     4801 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     4861 cgggagcaga caagcccgtc agggcgcg
//
