LOCUS       sequence-62152-e        4876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized 
            MaxGFP with Kan selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yoMaxGFP-Kan
FEATURES             Location/Qualifiers
     source          1..4876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     78..97
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        178..196
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     178..195
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      985..1172
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1040..1064)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            1247..2603
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1247..1590
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1591..2400
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1658..1677)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2406..2603
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2707..2726)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2708..2726)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2813..2830)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2984..3572)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3064..3083)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3743..4534,4535..4603))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4366..4385
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4604..4708)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4776..4794
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4832..4854)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
       61 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
      121 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
      181 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
      241 cggtgacggt gctggtttaa ttaacatgga atcagatgaa tctggattgc ctgccatgga
      301 aatcgaatgt cgaataacag gcacattaaa cggagtagaa tttgagttag tgggtggcgg
      361 tgaaggaacc ccagagcagg gtagaatgac taataagatg aaatccacca aaggcgctct
      421 tactttttct ccttatcttt tgagtcatgt tatgggttac gggttttatc attttggaac
      481 ttacccttct ggttatgaaa atccattcct gcacgccata aacaatggcg gttacacaaa
      541 cacgagaatt gaaaagtatg aagatggagg ggtcctccac gtgtcttttt catacagata
      601 cgaggctggt agggttatcg gggactttaa ggtaatgggt acaggctttc cagaagattc
      661 tgttattttc acagacaaaa tcatcagatc caatgcaaca gttgaacatc tacaccctat
      721 gggtgacaac gatttggacg gtagtttcac tcgtacattc tccttgagag atgggggtta
      781 ctacagctca gtcgtcgatt cacacatgca tttcaaatct gctattcatc catcaattct
      841 acaaaatggc ggtccaatgt ttgcttttag aagagtagaa gaggatcatt ctaatactga
      901 attaggcatc gttgagtacc aacatgcatt caaaactcca gacgcggatg caggagagga
      961 ataaggcgcg ccacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa
     1021 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
     1081 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagtat
     1141 gaggtcgctc ttattgacca cacctctacc ggcagatccg ctagggataa cagggtaata
     1201 tagatctgtt tagcttgcct cgtccccgcc gggtcacccg gccagcgaca tggaggccca
     1261 gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc
     1321 gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg
     1381 cacggcgcga agcaaaaatt acggctcctc gctgcagacc tgcgagcagg gaaacgctcc
     1441 cctcacagac gcgttgaatt gtccccacgc cgcgcccctg tagagaaata taaaaggtta
     1501 ggatttgcca ctgaggttct tctttcatat acttcctttt aaaatcttgc taggatacag
     1561 ttctcacatc acatccgaac ataaacaacc atgggtaagg aaaagactca cgtttcgagg
     1621 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     1681 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg
     1741 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     1801 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     1861 gatgcatggt tactcaccac tgcgatcccc ggcaaaacag cattccaggt attagaagaa
     1921 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     1981 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     2041 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2101 tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca
     2161 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2221 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2281 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     2341 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2401 tcagtactga caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt
     2461 atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg
     2521 acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta
     2581 tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta acgccgccat ccagtttaaa
     2641 cgagctcgaa ttcatcgatg atatcagatc cactagtggc ctatgcggcc gcggatctgc
     2701 cggtctccct atagtgagtc gtattaattt cgataagcca ggttaacctg cattaatgaa
     2761 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2821 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2881 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2941 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3001 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3061 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3121 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     3181 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3241 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3301 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3361 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3421 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3481 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3541 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3601 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3661 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3721 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3781 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3841 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3901 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3961 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4021 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4081 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4141 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4201 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4261 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4321 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4381 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4441 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4501 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4561 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4621 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4681 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     4741 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     4801 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     4861 tcacagcttg tctgta
//
