LOCUS       sequence-62158-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     Formosa lab yeast 3' tagging constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTF272 (pFA6a-TEV-6xGly-3xFlag-HphMX)
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..57
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             85..150
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      179..366
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(234..258)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        441..784
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta attaacgaga atttatactt ccaaggttct
       61 ggtggaggcg gtggaggctc tagagactac aaggaccacg acggtgacta caaggaccac
      121 gacattgact acaaggatga cgatgacaag tgactcgagg cgcgccactt ctaaataagc
      181 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata
      241 caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt
      301 cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc gctcttattg accacacctc
      361 taccggcaga tccgctaggg ataacagggt aatatagatc tgtttagctt gcctcgtccc
      421 cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg
      481 tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg
      541 tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct
      601 cctcgctgcg gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc
      661 acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc
      721 atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac
      781 aaccatgggt aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
      841 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
      901 cttc
//
