LOCUS       sequence-62161-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Burrows AE, Smogorzewska A, Elledge SJ
  TITLE     Polybromo-associated BRG1-associated factor components BRD7 and 
            BAF180 are critical regulators of p53 required for induction of 
            replicative senescence.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14280-5. doi: 
            10.1073/pnas.1009559107. Epub 2010 Jul 26.
  PUBMED    20660729
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1009559107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-08-10- 10"; volume: "107"; issue: "32"; pages: "14280-5"
COMMENT     Sequence Label: MSCV-PM-BAF180-SH2
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           64..185
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           213..227
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           255..381
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(475..494)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(476..494)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(548..571)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     945..964
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt agatctctcg actagggata acagggtaat
       61 tgtttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg gaaacacttg
      121 ctgggattac ttcttcaggt taacccaaca gaaggctcga gaagatatat tgctgttgac
      181 agtgagcgcc gctaccgtcg gcttgattta tagtgaagcc acagatgtat aaatcaagcc
      241 gacggtagcg atgcctactg cctcggaatt caaggggcta ctttaggagc aattatcttg
      301 tttactaaaa ctgaatacct tgctatctct ttgatacatt tttacaaagc tgaattaaaa
      361 tggtataaat taaatcactt ttttcaattg gaagactaat gcggccggcc agcttgttgg
      421 gttctctttc tgcgtggcaa tgggggtgtt cgttattttt cattctcgtt tgggccctat
      481 agtgagtcgt attacctagg acgcgttcta ccgggtaggg gaggcgcttt tcccaaggca
      541 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
      601 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
      661 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
      721 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
      781 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
      841 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
      901 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
      961 aaagcgcacg tctgccgcgc tgttc
//
