LOCUS       sequence-62163-e        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Burrows AE, Smogorzewska A, Elledge SJ
  TITLE     Polybromo-associated BRG1-associated factor components BRD7 and 
            BAF180 are critical regulators of p53 required for induction of 
            replicative senescence.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14280-5. doi: 
            10.1073/pnas.1009559107. Epub 2010 Jul 26.
  PUBMED    20660729
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1009559107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-08-10- 10"; volume: "107"; issue: "32"; pages: "14280-5"
COMMENT     Sequence Label: MSCV-PM BRD7 sh2
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           66..187
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           215..229
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           257..383
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        433..932
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(468..491)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     865..884
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(953..972)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatctct cgactaggga taacagggta
       61 attgtttgaa tgaggcttca gtactttaca gaatcgttgc ctgcacatct tggaaacact
      121 tgctgggatt acttcttcag gttaacccaa cagaaggctc gagaaggtat attgctgttg
      181 acagtgagcg catggatttt agtaccatga aatagtgaag ccacagatgt atttcatggt
      241 actaaaatcc atttgcctac tgcctcggaa ttcaaggggc tactttagga gcaattatct
      301 tgtttactaa aactgaatac cttgctatct ctttgataca tttttacaaa gctgaattaa
      361 aatggtataa attaaatcac ttttttcaat tggaagacta atgcgtttaa acacgcggcg
      421 acgcgttcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
      481 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
      541 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
      601 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
      661 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
      721 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
      781 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
      841 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
      901 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
      961 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg ta
//
