LOCUS       sequence-62164-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Burrows AE, Smogorzewska A, Elledge SJ
  TITLE     Polybromo-associated BRG1-associated factor components BRD7 and 
            BAF180 are critical regulators of p53 required for induction of 
            replicative senescence.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14280-5. doi: 
            10.1073/pnas.1009559107. Epub 2010 Jul 26.
  PUBMED    20660729
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1009559107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-08-10- 10"; volume: "107"; issue: "32"; pages: "14280-5"
COMMENT     Sequence Label: MSCV-PM BRD7 sh1
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           66..187
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           215..229
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           257..383
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(476..495)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(477..495)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(549..572)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatctct cgactaggga taacagggta
       61 attgtttgaa tgaggcttca gtactttaca gaatcgttgc ctgcacatct tggaaacact
      121 tgctgggatt acttcttcag gttaacccaa cagaaggctc gagaaggtat attgctgttg
      181 acagtgagcg caggatgatt ctgatttaat cttagtgaag ccacagatgt aagattaaat
      241 cagaatcatc ctttgcctac tgcctcggaa ttcaaggggc tactttagga gcaattatct
      301 tgtttactaa aactgaatac cttgctatct ctttgataca tttttacaaa gctgaattaa
      361 aatggtataa attaaatcac ttttttcaat tggaagacta atgcggccgg ccatatttcg
      421 gtgtgtctac gtttatttta tggtctagtt tcgattttgt tttcccattt gtgggcccta
      481 tagtgagtcg tattacctag gacgcgttct accgggtagg ggaggcgctt ttcccaaggc
      541 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
      601 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
      661 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
      721 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
      781 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
      841 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
      901 ggctcagggg cggggcgggc gccc
//
