LOCUS       sequence-62168-e        5160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Lane MC, Alteri CJ, Smith SN, Mobley HL
  TITLE     Expression of flagella is coincident with uropathogenic Escherichia 
            coli ascension to the upper urinary tract.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16669-74. Epub 2007 
            Oct 9.
  PUBMED    17925449
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-10-16"; volume: "104(42)"; pages: 
            "16669-74. Epub 2007 Oct 9"
COMMENT     Sequence Label: pGEN-MCS
FEATURES             Location/Qualifiers
     source          1..5160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..152
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     rep_origin      complement(283..828)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(363..382)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(903..930)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1022..1108)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1261..1278
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(join(1823..2614,2615..2683))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2446..2465
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2684..2788)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2856..2874
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattcctgc agggcatgcc ccgggcatat gaagcttggt accgggatcc gcggccgcgg
       61 gccccatgga tcgatagctg gtcgacacaa tctgcccttt cgaaagatcc caacgaaaag
      121 cgtgaccaca tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat
      181 gagctctaca aataatgagc tagcccgcct aatgagcggg cttttttttc tcggcctagg
      241 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
      301 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
      361 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
      421 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
      481 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
      541 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
      601 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
      661 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
      721 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
      781 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt
      841 attaatcaga taaaatattt ctaggatcta aaacactagg cccaagagtt tgtagaaacg
      901 caaaaaggcc atccgtcagg atggccttct gcttaatttg atgcctggca gtttatggcg
      961 ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc gctcccggcg
     1021 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     1081 ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcatgggga
     1141 gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt
     1201 gggaccaccg cgctactgcc gccaggcaaa ttctgtttta tcagaccgct tctgcgttct
     1261 gatttaatct gtatcaggct gaaaatcttc tctcatccgc caaaacagcc aagctggatc
     1321 tggcaaatcg ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt
     1381 ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact
     1441 acaggcgcct tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca
     1501 cgggcttctc agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg
     1561 ttcagcagtt cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg
     1621 tcattcagac tggctaatgc acccagtaag gcagcggtat catcaacagg cttacccgtc
     1681 ttactgtcaa ccggatctaa aacactagtt aagggatttt ggtcatgaga ttatcaaaaa
     1741 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1801 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     1861 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     1921 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     1981 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2041 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2101 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2161 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2221 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2281 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2341 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2401 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     2461 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2521 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2581 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2641 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2701 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2761 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     2821 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     2881 gtcttctaga caacatcagc aaggagaaag gggctaccgg cgaaccagca gcccctttat
     2941 aaaggcgctt cagtagtcag accagcatca gtcctgaaaa ggcgggcctg cgcccgcctc
     3001 caggttgcta cttaccggat tcgtaagcca tgaaagccgc cacctccctg tgtccgtctc
     3061 tgtaacgaat ctcgcacagc gattttcgtg tcagataagt gaatatcaac agtgtgagac
     3121 acacgatcaa cacacaccag acaagggaac ttcgtggtag tttcatggcc ttcttctcct
     3181 tgcgcaaagc gcggtaagag gctatcctga tgtggactag acatagggat gcctcgtggt
     3241 ggttaatgaa aattaactta ctacggggct atcttctttc tgccacacaa cacggcaaca
     3301 aaccaccttc acgtcatgag gcagaaagcc tcaagcgccg ggcacatcat agcccatata
     3361 cctgcacgct gaccacactc actttccctg aaaataatcc gctcattcag accgttcacg
     3421 ggaaatccgt gtgattgttg ccgcatcacg ctgcctcccg gagtttgtct cgagcacttt
     3481 tgttacccgc caaacaaaac ccaaaaacaa cccataccca acccaataaa acaccaaaac
     3541 aagacaaata atcattgatt gatggttgaa atggggtaaa cttgacaaac aaacccactt
     3601 aaaacccaaa acatacccaa acacacacca aaaaaacacc ataaggagtt ttataaatgt
     3661 tggtattcat tgatgacggt tcaacaaaca tcaaactaca gtggcaggaa agcgacggaa
     3721 caattaaaca gcacattagc ccgaacagct tcaaacgcga gtgggcagtc tcttttggtg
     3781 ataaaaaggt ctttaactac acactgaacg gcgaacagta ttcatttgat ccaatcagcc
     3841 cggatgctgt agtcacaacc aatatcgcat ggcaatacag cgacgttaat gtcgttgcag
     3901 tgcatcacgc cttactgacc agtggtctgc cggtaagcga agtggatatt gtttgcacac
     3961 ttcctctgac agagtattac gacagaaata accaacccaa tacggaaaat attgagcgta
     4021 agaaagcaaa cttccggaaa aaaattacat taaatggcgg ggatacattc acaataaaag
     4081 atgtaaaagt catgcctgaa tctataccgg caggttatga agttctacaa gaactggatg
     4141 agttagattc tttattaatt atagatctcg ggggcaccac attagatatt tctcaggtaa
     4201 tggggaaatt atcggggatc agtaaaatat acggagactc atctcttggt gtctctctgg
     4261 ttacatctgc agtaaaagat gccctttctc ttgcgagaac aaaaggaagt agctatcttg
     4321 ctgacgatat aatcattcac agaaaagata ataactatct gaagcaacga attaatgatg
     4381 agaacaaaat atcaatagtc accgaagcaa tgaatgaagc acttcgtaaa cttgagcaac
     4441 gtgtattaaa tacgctcaat gaattttctg gttatactca tgttatggtt ataggcggtg
     4501 gcgcagaatt aatatgcgat gcagtaaaaa aacacacaca gattcgtgat gaacgttttt
     4561 tcaaaaccaa taactctcaa tatgatttag ttaacggtat gtatctcata ggtaattaat
     4621 gatggacaag cgcagaacca ttgccttcaa actaaatcca gatgtaaatc aaacagataa
     4681 aattgtttgt gatacactgg acagtatccc gcaaggggaa cgaagccgcc ttaaccgggc
     4741 cgcactgacg gcaggtctgg ccttatacag acaagatccc cggacccctt tccttttatg
     4801 tgagctgctg acgaaagaaa ccacattttc agatatcgtg aatatattga gatcgctatt
     4861 tccaaaagag atggccgatt ttaattcttc aatagtcact caatcctctt cacaacaaga
     4921 gcaaaaaagt gatgaagaga ccaaaaaaaa tgcgatgaag ctaataaatt aattcaatta
     4981 ttattgagtt ccctttatcc actatcaggc tggataaagg gaactcaatc aagttatttt
     5041 cttaccagtc attacataat cgttattatg aaataatcgt ttgcactgtc tctgttattc
     5101 aggcaatttc aataaaggca cttgctcacg ctctgtcatt ttctgaaact cttcatgctg
//
