LOCUS       sequence-62174-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: IV-GCA
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(475..493)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(476..493)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(511..527)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(511..527)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(524..546)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    535..551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(559..565,566..583,584..589))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    604..625
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(742..759)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
       61 ctgcagaatt cgcccttagt atctttcctg tgcccacgtc tctctccagt gcgggtctcc
      121 cgccaatgtt ggaggcgatt gccacgacct gctctggggt taatccgtgc gcttggcaca
      181 gcacaggcaa cagcctctgt actgtttcca aggcttgctt tcctccgtcg tggcttgcga
      241 tcgccacgac ctgctctggg gtgaggccgt gcgcttggca cagcactgga aggagcctct
      301 gcaccgtttc caaagcctgt tttccgccgt tattgctcgc gattgccacg acctgctctg
      361 gggtgagtcg agacgcgcta ggacggcatc tatataaggg cgaattccag cacactggcg
      421 gccgttacta gtggatccga gctcggtacc aagcttgatg catagcttga gtattctata
      481 gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      541 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      601 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      661 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      721 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      781 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      841 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc agg
//
