LOCUS       sequence-62204-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  TITLE     pET15b-At 14-3-3 omicron
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET15b-At 14-3-3 omicron
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggagaacg agagagcgaa gcaagtgtac ttggcgaaac tcaacgagca agctgagaga
      121 tacgatgaga tggttgaagc aatgaagaaa gttgcagctc ttgatgtaga gctcaccatt
      181 gaagagagga atcttttatc tgttggttac aaaaatgtga ttggtgctag gagagcatcg
      241 tggaggatcc tttcttcgat cgagcagaaa gaagaatcga aaggaaacga acaaaatgcc
      301 aagaggatta aggattacag gacaaaagtt gaagaagaac tctccaagat ttgttacgat
      361 attttggctg tcatcgataa gcatcttgtc ccattcgcaa cttcgggaga atccaccgtc
      421 ttctactaca aaatgaaagg agattatttc agatacttgg ctgaatttaa gtccggtgct
      481 gatcgcgagg aagctgcgga cctatcactc aaggcgtatg aagctgcaac gagttctgct
      541 agcacggaat tgtctacaac acatccaata agactcggct tggctctcaa cttttctgtc
      601 ttctactacg agatcttgaa ttctccggaa agagcatgtc atttggcaaa gcgagcgttt
      661 gatgaagcaa tcgctgagct tgacagtctt aacgaagatt catacaagga cagcacactt
      721 atcatgcaac ttctcagaga taacctcact ttatggacct ctgatctaga ggaaggaggt
      781 gaacaatcta aaggccataa tcaacaagat gagaactaac tcgaggatcc ggctgctaac
      841 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataa
//
