LOCUS       sequence-62219-e        4894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized 
            photoconvertible CFP2 with Kan selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yoPS-CFP2-Kan
FEATURES             Location/Qualifiers
     source          1..4894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(34..56)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     156..175
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        256..274
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     256..273
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1081..1268
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1136..1160)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            1343..2699
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1343..1686
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1687..2496
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1754..1773)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2502..2699
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2803..2822)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2804..2822)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2909..2926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3080..3668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3160..3179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3839..4630,4631..4699))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4462..4481
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4700..4804)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4872..4890
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
       61 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      121 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
      181 agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca taaccttatg
      241 tatcatacac atacgattta ggtgacacta tagaacgcgg ccgccagctg aagcttcgta
      301 cgctgcaggt cgacggatcg gtgacggtgc tggtttaatt aacatgtcca aaggagccga
      361 attgtttaca ggtattgttc ctatcctcat agaattgaac ggtgatgtaa acggtcataa
      421 gttttctgtc agcggagaag gtgaagggga tgctacctat ggtaaactaa ctctgaaatt
      481 catttgtaca accggcaagt tgccagttcc ttggcctaca ttagtggcta cattatcata
      541 cggggttcag tgcttttcta gatatccaga ccacatgaaa caacatgatt tctttaagtc
      601 agcaatgcca gaaggataca tccaagagag aaccatcttt ttcgaagatg atggcaatta
      661 caagacacgt gcggaagtta agttcgaagg tgatacgcta gtgtccagga ttgaacttac
      721 aggaacagat ttcaaagagg atggcaatat cctaggcaac aaaatggagt ataactacaa
      781 tgcaactaat gtctatatcg ttgcagacaa ggccagaaat ggtattaaag taaactttaa
      841 ggtaagacat aacataaaag atggatctgt ccaattggca gaccattacc aacagaatac
      901 tcctattggt gacggtccag tgctgcttcc agataatcac tacttatcta ctcaatcagc
      961 cttgagtaaa gacccaaatg aaaagagaga tcacatgatc taccttgagt ttgttactgc
     1021 tgctgctata actcatggca tggacgaatt atacaaataa ggcgcgccac ttctaaataa
     1081 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta
     1141 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct
     1201 ttcctgtagg tcaggttgct ttctcaggta tagtatgagg tcgctcttat tgaccacacc
     1261 tctaccggca gatccgctag ggataacagg gtaatataga tctgtttagc ttgcctcgtc
     1321 cccgccgggt cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga
     1381 cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca
     1441 tgtataatca tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg
     1501 ctcctcgctg cagacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc
     1561 ccacgccgcg cccctgtaga gaaatataaa aggttaggat ttgccactga ggttcttctt
     1621 tcatatactt ccttttaaaa tcttgctagg atacagttct cacatcacat ccgaacataa
     1681 acaaccatgg gtaaggaaaa gactcacgtt tcgaggccgc gattaaattc caacatggat
     1741 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1801 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1861 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1921 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1981 atccccggca aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2041 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2101 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2161 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2221 gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2281 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2341 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2401 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2461 ttgcagtttc atttgatgct cgatgagttt ttctaatcag tactgacaat aaaaagattc
     2521 ttgttttcaa gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat
     2581 caaatgttag cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt
     2641 taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc
     2701 tgtcgattcg atactaacgc cgccatccag tttaaacgag ctcgaattca tcgatgatat
     2761 cagatccact agtggcctat gcggccgcgg atctgccggt ctccctatag tgagtcgtat
     2821 taatttcgat aagccaggtt aacctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2881 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2941 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3001 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3061 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3241 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     3301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3481 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     3541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3721 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3781 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3841 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3901 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3961 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4021 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4081 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4141 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4201 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4261 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4321 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4381 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4441 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4501 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4561 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4621 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4681 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4741 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4801 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     4861 taacctataa aaataggcgt atcacgaggc cctt
//
