LOCUS       sequence-62223-e        4888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast optimized 
            photoconvertible mOrange with Kan selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Lee S, Lim WA, Thorn KS
  TITLE     Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors 
            for S. cerevisiae.
  JOURNAL   PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902.
            Print 2013.
  PUBMED    23844123
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-2"; volume: "8(7):e67902. doi"; pages: " 
            10.1371/journal.pone.0067902. Print 2013"
COMMENT     Sequence Label: pFA6a-link-yoPSmOrange-Kan
FEATURES             Location/Qualifiers
     source          1..4888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3..25)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     125..144
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        225..243
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     225..242
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      1044..1231
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1099..1123)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            1306..2662
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1306..1649
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1650..2459
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1717..1736)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2465..2662
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2766..2785)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2767..2785)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2872..2889)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3043..3631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3123..3142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3802..4593,4594..4662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4425..4444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4663..4767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4835..4853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
       61 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
      121 tatgcggcat cagagcagat tgtactgaga gtgcaccata tggacatatt gtcgttagaa
      181 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
      241 agaacgcggc cgccagctga agcttcgtac gctgcaggtc gacggatcgg tgacggtgct
      301 ggtttaatta acatggtgtc taaaggtgag gaaaacaaca tggcaatcat taaggaattc
      361 atgagattca aagtcagaat ggaagggaca gttaatggcc acgagtttga aatcgaaggc
      421 gaaggtgaag gtcgtccata cgagggtttt caaacagcta agttaaaagt gacgaaagga
      481 ggaccactac cattcgcttg ggatattctc agtccattgt ttacttatgg gtcaaaagct
      541 tacgttaaac atcctgccga catccctgac tactttaagt tgtccttccc agaagggttt
      601 aagtgggaaa gagttatgaa ttatgaggac ggaggtgttg taactgtcac ccaagactct
      661 tctcttcaag atggtgaatt catctacaaa gttaagatga gaggcacaaa ttttccaagc
      721 gatggcccag ttatgcagaa aaagacaatg ggctgggagg catcctcaga gaggatgtac
      781 cctgaagatg gagcgttaaa gggagagata cgaatgagac tgaagttaaa ggatggaggt
      841 cattacacta gtgaagtaaa aactacttat aaagctaaaa agtctgtaca acttcctggc
      901 gcatatattg tgggtattaa actagatata acatcacaca atgaagatta caccatcgtc
      961 gaacaatacg aaagagccga aggtagacat tctacaggtg gtatggatga attgtacaaa
     1021 taaggcgcgc cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat
     1081 aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa
     1141 aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagtatg
     1201 aggtcgctct tattgaccac acctctaccg gcagatccgc tagggataac agggtaatat
     1261 agatctgttt agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag
     1321 aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg
     1381 tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc
     1441 acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc
     1501 ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag
     1561 gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt
     1621 tctcacatca catccgaaca taaacaacca tgggtaagga aaagactcac gtttcgaggc
     1681 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1741 tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt
     1801 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1861 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1921 atgcatggtt actcaccact gcgatccccg gcaaaacagc attccaggta ttagaagaat
     1981 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     2041 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     2101 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     2161 ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca ccggattcag
     2221 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     2281 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     2341 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     2401 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     2461 cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta
     2521 tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga
     2581 catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat
     2641 gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtttaaac
     2701 gagctcgaat tcatcgatga tatcagatcc actagtggcc tatgcggccg cggatctgcc
     2761 ggtctcccta tagtgagtcg tattaatttc gataagccag gttaacctgc attaatgaat
     2821 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2881 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2941 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3001 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3061 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     3241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3481 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     4081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     4141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     4441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     4681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     4741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     4801 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     4861 tctcgcgcgt ttcggtgatg acggtgaa
//
