LOCUS       sequence-62224-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Ding Q, Lee YK, Schaefer EA, Peters DT, Veres A, Kim K, Kuperwasser 
            N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan
            A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa 
            E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung 
            RT, Musunuru K, Cowan CA
  TITLE     A TALEN Genome-Editing System for Generating Human Stem Cell-Based 
            Disease Models.
  JOURNAL   Cell Stem Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011.
  PUBMED    23246482
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2012 Dec 12. pii: S1934-5909(12)00645-5. doi: 
            10.1016/j.stem.2012.11.011."
COMMENT     Sequence Label: IV-ACC
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(476..494)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(477..494)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(512..528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(512..528)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(525..547)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    536..552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(560..566,567..584,585..590))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    605..626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(743..760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
       61 ctgcagaatt cgcccttaag tatctttcct gtgcccacgt ctctctccag tgcgggtctt
      121 cctccgtcgt ggcttgcgat cgccacgacc tgctctgggg ttaatccgtg cgcttggcac
      181 agcacaggca acagcctctg tactgtttcc aaggcttgct ttcctccgtc gtggcttgcg
      241 atcgccacga cctgctctgg ggtgaggccg tgcgcttggc acagcacggg aagcaggcgc
      301 tggacagtct cgagtgcctg tttcccgcca atgttggagg cgattgccac gacctgctct
      361 ggggtgagtc gagacgcgct aggacggcat ctatataagg gcgaattcca gcacactggc
      421 ggccgttact agtggatccg agctcggtac caagcttgat gcatagcttg agtattctat
      481 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      541 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      601 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      661 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      721 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      781 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      841 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      901 cgttgctggc gtttttccat aggctc
//
