LOCUS       sequence-62241-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Landthaler M, Yalcin A, Tuschl T
  TITLE     The human DiGeorge syndrome critical region gene 8 and Its D. 
            melanogaster homolog are required for miRNA biogenesis.
  JOURNAL   Curr Biol. 2004 Dec 14. 14(23):2162-7.
  PUBMED    15589161
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2004 Dec 14. 14(23):2162-7."
COMMENT     Sequence Label: pFLAG/HA-DGCR8
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             148..171
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             172..198
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    214..238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagcttggt accgagctcg gatcgatcca tcgatagtgt
      121 ggtggaattc tgatcgccgc cgccatggac tacaaggacg acgatgacaa gtacccttat
      181 gacgtgcccg attacgcttt gaaccagata tcaacaagtt tgtacaaaaa agcaggctcc
      241 accatgggaa ccaattcagt cgacatggag acagatgaga gcccctctcc gctcccgtgt
      301 gggcccgcag gagaagcggt gatggagagc cgagctcgcc ccttccaagc gctgccccgt
      361 gagcagtctc caccacctcc cctgcaaacg tccagtggtg cagaggtaat ggacgttggc
      421 tctggtggtg atggacagtc cgaactccct gctgaggacc ccttcaactt ctacggagct
      481 tctcttctct ccaaaggatc cttctctaag ggccgcctcc tcatagaccc gaactgtagt
      541 ggccacagcc cgcgcaccgc ccggcacgca cctgcggtcc ggaagttctc ccctgacctt
      601 aagttgctta aggatgtaaa gattagcgtg agctttaccg agagctgcag gagtaaggac
      661 aggaaggtgc tgtacacagg agcagagcgc gacgtgcggg cggagtgcgg tctgctcctt
      721 agccctgtca gtggggacgt gcatgcttgt ccctttggcg ggagtgttgg tgacggggta
      781 ggcatagggg gtgagagtgc tgataagaag gatgaggaga atgagctgga tcaggaaaag
      841 agagtggagt atgcagtgct cgatgagtta gaagatttta ctgacaattt ggagctagat
      901 gaagaaggag ca
//
