LOCUS       sequence-62251-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human Bip with Myc tag near the C-terminus, 
            retaining the C-terminal KDEL ER localization sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Shen J, Chen X, Hendershot L, Prywes R
  TITLE     ER stress regulation of ATF6 localization by dissociation of 
            BiP/GRP78 binding and unmasking of Golgi localization signals.
  JOURNAL   Dev Cell. 2002 Jul . 3(1):99-111.
  PUBMED    12110171
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2002 Jul . 3(1):99-111."
COMMENT     Sequence Label: pCMV BiP-Myc-KDEL-wt
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(194..259)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(293..322)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ggttgtcttc ccaacttgcc ccttgctcca taccaccccc ctccacccca taatattata
       61 gaaggacacc tagtcagaca aaatgatgca acttaatttt attaggacaa ggctggtggg
      121 cactggagtg gcaacttcca gggccaggag aggcactggg gaggggtcac agggatgcca
      181 cccgggatca ctacttgtca tcgtcatcct tgtagtcgat gtcatgatct ttataatcac
      241 cgtcatggtc tttgtagtca gcccgggatc ctctagacta cagctcgtcc ttcagatcct
      301 cttctgagat gagtttttgt tcgaagggcc ctctagccaa ctcatctttt tctgctgtat
      361 cctcttcacc agttggggga gggcctgcac ttccatagag tttgctgata attggttgaa
      421 caatttcttc cagttccttc ttcttagctt tgaagtcttc aatgtcagca tcttggtggc
      481 tttccagcca ttcaatcttt tcttctacag ctttttccat ggtctcctta tcttcagagg
      541 aaagtttacc tcccagcttt tctttatctc caatctgatt ctttagagaa taggcatagc
      601 tttccaactc atttctagta tcaatgcgct ccttgagctt tttgtcttcc tcagcaaact
      661 tctcagcatc attaaccatc ctttcgattt cttcaggtgt caggcgattc tggtcattgg
      721 tgattgtgat cttatttttg ttccctgtac ccttgtcttc agctgtcact cgaagaatac
      781 cattcacatc tatctcaaag gtgacttcaa tctgtgggac cccacgagga gcaggaggaa
      841 ttccagtcag atcaaatgta cccagaagat gattgtcttt tgtcaggggt ctttcacctt
      901 catagacctt gattgtaaca gttggt
//
