LOCUS       sequence-62261-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of ultrasensitive protein calcium sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, Baohan A, 
            Schreiter ER, Kerr RA, Orger MB, Jayaraman V, Looger LL, Svoboda K, 
            Kim DS
  TITLE     Ultrasensitive fluorescent proteins for imaging neuronal activity.
  JOURNAL   Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.
  PUBMED    23868258
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2013-07-18- 18"; volume: "499"; issue: "7458"; 
            pages: "295-300"
COMMENT     Sequence Label: pGP-CMV-GCaMP6m
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     704..723
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     963..984
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatct agagtcgcgg ccgctcactt cgctgtcatc atttgtacaa actcttcgta
      181 gtttacctga ccatccccat cgatgtctgc ttccctgatc atttcatcaa cctcttcatc
      241 tgttaacttc tctccaaggt ttgtcatcac gtggcgaagc tctgctgcac tgatgtagcc
      301 attgccatcc ttatcaaaca caccgaacgc ttctctaatt tcttcttccg tgtccctgta
      361 gctccctttt cttgccatca ttgtcaggaa ctcagggaag tcgattgtgc cgtcaccgtc
      421 ggcatctact tcattgatca tgtcctgcag ctctgcttct gtggggttct gccccagaga
      481 ccgcatcacc gtccccagct ccttggttgt tattgtccca tccccgtcct tgtcaaatag
      541 ggagaaagcc tctttaaatt ctgcgatctg ctcttcagtc agttggtccg gcaggttgta
      601 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      661 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      721 gtccttgaag aagatggtgc gctcctggat gtagccttcg ggcatggcgg acttgaagaa
      781 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
      841 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
      901 cagcttgccg taggtggcat cgccctcacc ctcgccggac acgctgaact tgtggccgtt
      961 tacgtcgccg tccagctcga ccaggatggg caccacccc
//
