LOCUS       sequence-62265-e        6055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Crefcoeur RP, Yin R, Ulm R, Halazonetis TD
  TITLE     Ultraviolet-B-mediated induction of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800.
  PUBMED    23653191
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1779. doi"; pages: " 
            10.1038/ncomms2800"
COMMENT     Sequence Label: pSNV2 CHER NLS cop1at(335-675)
FEATURES             Location/Qualifiers
     source          1..6055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        107..211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(212..280,281..1072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(430..449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1243..1831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1732..1751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1985..2002
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2017..2157
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2083..2102)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2243..2265
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2303..2323)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2308..2641
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2488..2627
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2657..3364
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2802..2820)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3070..3089
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3377..3397
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4531..4596
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4726..4746
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5171..5305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5208..5227)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5262..5281
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      421 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
      481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      721 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
      781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1081 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1141 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1201 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1261 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1321 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1381 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1441 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1501 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1561 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1621 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1681 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1741 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1801 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1861 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1921 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     1981 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     2041 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     2101 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     2161 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     2221 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     2281 ccgaaacgcg cgaggcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     2341 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     2401 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2461 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     2521 tctccgcccc atgcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     2581 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     2641 aaagcttgcc gccacgatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     2701 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     2761 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     2821 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     2881 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     2941 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     3001 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     3061 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     3121 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     3181 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     3241 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     3301 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     3361 caagggtggt aaaggaccta aaaagaaacg taaggtcgga cccgggtata gcaacggcct
     3421 tgcagatttt caatctgtgt tgactacctt cactcgctac agtcgtctaa gagttatagc
     3481 agaaatccgg catggggata tatttcattc agccaacatt gtatcaagca tagagtttga
     3541 tcgtgatgat gagctgtttg ccactgctgg tgtttctaga tgtataaagg tttttgactt
     3601 ctcttcggtt gtaaatgaac cagcagatat gcagtgtccg attgtggaga tgtcaactcg
     3661 gtctaaactt agttgcttga gttggaataa gcatgaaaaa aatcacatag caagcagtga
     3721 ttatgaagga atagtaacag tgtgggatgt aactactagg cagagtctta tggagtatga
     3781 agagcacgaa aaacgtgcct ggagtgttga cttttcacga acagaaccat caatgcttgt
     3841 atctggtagt gacgactgca aggttaaagt ttggtgcacg aggcaggaag caagtgtgat
     3901 taatattgat atgaaagcaa acatatgttg tgtcaagtac aatcctggct caagcaacta
     3961 cattgcggtc ggatcagctg atcatcacat ccattattac gatctaagaa acataagcca
     4021 accacttcat gtcttcagtg gacacaagaa agcagtttcc tatgttaaat ttttgtccaa
     4081 caacgagctc gcttctgcgt ccacagatag cacactacgc ttatgggatg tcaaagacaa
     4141 cttgccagtt cgaacattca gaggacatac taacgagaag aactttgtgg gtctcacagt
     4201 gaacagcgag tatctcgcct gtggaagcga gacaaacgaa gtatatgtat atcacaagga
     4261 aatcacgaga cccgtgacat cgcacagatt tggatcgcca gacatggacg atgcagagga
     4321 agaggcaggt tcctacttta ttagtgcggt ttgctggaag agtgatagtc ccacgatgtt
     4381 gactgcgaat agtcaaggaa ccatcaaagt tctggtactc gctgcgtaag cggccgcaaa
     4441 agtcgacctg aagctatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     4501 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     4561 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     4621 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     4681 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     4741 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     4801 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     4861 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     4921 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     4981 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     5041 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     5101 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     5161 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5221 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5281 caatgtatct tatcatgtct ggatcgatcc ccaggaagct cctctgtgtc ctcataaacc
     5341 ctaacctcct ctacttgaga ggacattcca atcataggct gcccatccac cctctgtgtc
     5401 ctcctgttaa ttaggtcact taacaaaaag gaaattgggt aggggttttt cacagaccgc
     5461 tttctaaggg taattttaaa atatctggga agtcccttcc actgctgtgt tccagaagtg
     5521 ttggtaaaca gcccacaaat gtcaacagca gaaacataca agctgtcagc tttgcacaag
     5581 ggcccaacac cctgctcatc aagaagcact gtggttgctg tgttagtaat gtgcaaaaca
     5641 ggaggcacat tttccccacc tgtgtaggtt ccaaaatatc tagtgttttc atttttactt
     5701 ggatcaggaa cccagcactc cactggataa gcattatcct tatccaaaac agccttgtgg
     5761 tcagtgttca tctgctgact gtcaactgta gcattttttg gggttacagt ttgagcagga
     5821 tatttggtcc tgtagtttgc taacacaccc tgcagctcca aaggttcccc accaacagca
     5881 aaaaaatgaa aatttgaccc ttgaatgggt tttccagcac cattttcatg agttttttgt
     5941 gtccctgaat gcaagtttaa catagcagtt accccaataa cctcagtttt aacagtaaca
     6001 gcttcccaca tcaaaatatt tccacaggtt aagtcctcat ttaaattagg caaag
//
