LOCUS       sequence-62269-e        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Wu K, Lu G, Sehnke P, Ferl RJ
  TITLE     The heterologous interactions among plant 14-3-3 proteins and 
            identification of regions that are important for dimerization.
  JOURNAL   Arch Biochem Biophys. 1997 Mar 1;339(1):2-8.
  PUBMED    9056226
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Biochem Biophys."; date: "1997-03-1"; volume: "339(1)"; pages: "2-8"
COMMENT     Sequence Label: pET15b-At 14-3-3 omega
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     84..103
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        84..102
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    103..127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             143..165
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             184..201
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             211..228
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gatatagcgc agcacggcac ctgtggcgcc gtgatgcaca cgatgcgtcc ggcgtagaga
       61 tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
      121 caattcccct ctagaaaata attttgttta actttaagaa ggagatatac catgggcagc
      181 agccatcatc atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatggcgtct
      241 gggcgtgaag agttcgtata catggctaag ctcgcggagc aagcggagag gtacgaagag
      301 atggtagagt ttatggagaa agtctccgcc gccgttgatg gcgatgaact caccgtagaa
      361 gagcgaaatc ttctctccgt cgcttataag aatgtgattg gtgctcgccg tgcctcgtgg
      421 cgtatcattt catcgatcga gcagaaggaa gagagccgtg gtaacgatga ccacgtcacg
      481 gcgatccgtg aatataggtc taagatcgag acggaactct ccggaatctg cgacggaatc
      541 cttaagttgc ttgactctag actcatccct gccgctgctt ctggtgattc caaggtcttt
      601 taccttaaga tgaagggaga ttatcacagg tacttggctg agtttaagac tggtcaagag
      661 aggaaagacg ccgccgaaca tacactcgcc gcttacaaat ctgctcagga tattgctaat
      721 gcagagcttg ctccaacaca cccaattcgt cttggtcttg cattgaactt ctctgtgttc
      781 tattacgaga tcctcaattc tcctgatcgt gcctgtaacc tcgccaaaca ggcctttgat
      841 gaggcgattg cagagttgga cactcttggt gaagagtcat acaaagacag taccttgatc
      901 atgcagcttc ttcgtgacaa tctcactctc tggacatctg atatgcagga tgatgctgcg
      961 gatgagatca aggaagcagc agcgccaaaa ccgaccgagg aacagcagtg aattccaagc
     1021 cgaattccag cacactggcg gccgttacta gtggatccgg ctgctaacaa agcccgaaag
     1081 agctattc
//
