LOCUS       sequence-62271-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  TITLE     Plasmids to assay for nuclease cleavage in planta
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHEX2 T494 TALENs
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    43..67
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             89..154
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             161..181
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttatttgga gaggacacgc tcgaggaatt cggtacccca tcacaagttt gtacaaaaaa
       61 gcaggctccg aattcgccct tcaccatgga ttataaggat cacgatggcg actacaagga
      121 ccacgatatt gactacaaag acgatgacga taaaatggct cctaagaaaa agcgcaaagt
      181 cggtatccat ggcgttccct ctagaatggt ggatctacgc acgctcggct acagtcagca
      241 gcagcaagag aagatcaaac cgaaggtgcg ttcgacagtg gcgcagcacc acgaggcact
      301 ggtgggccat gggtttacac acgcgcacat cgttgcgctc agccaacacc cggcagcgtt
      361 agggaccgtc gctgtcacgt atcagcacat aatcacggcg ttgccagagg cgacacacga
      421 agacatcgtt ggcgtcggca aacagtggtc cggcgcacgc gccctggagg ccttgctcac
      481 ggatgcgggg gagttgagag gtccgccgtt acagttggac acaggccaac ttgtgaagat
      541 tgcaaaacgt ggcggcgtga ccgcaatgga ggcagtgcat gcatcgcgca atgcactgac
      601 gggtgccccc ctgaacctga ccccggacca agtggtggct atcgccagca acaatggcgg
      661 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
      721 gactccggac caagtggtgg ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac
      781 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
      841 ggctatcgcc agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
      901 ggtgctgtgc caggaccatg gcctgacccc ggaccaa
//
