LOCUS       sequence-62276-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a catalytically inactive, human codon-optimized 
            Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR
            promoter for mammalian gene knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim 
            WA
  TITLE     Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific 
            Control of Gene Expression.
  JOURNAL   Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
  PUBMED    23452860
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.02"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1173-83"
COMMENT     Sequence Label: pdCas9::BFP-humanized
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggaa ttagatctcg ccaccatgga caagaagtat
       61 tctatcggac tggccatcgg gactaatagc gtcgggtggg ccgtgatcac tgacgagtac
      121 aaggtgccct ctaagaagtt caaggtgctc gggaacaccg accggcattc catcaagaaa
      181 aatctgatcg gagctctcct ctttgattca ggggagaccg ctgaagcaac ccgcctcaag
      241 cggactgcta gacggcggta caccaggagg aagaaccgga tttgttacct tcaagagata
      301 ttctccaacg aaatggcaaa ggtcgacgac agcttcttcc ataggctgga agaatcattc
      361 ctcgtggaag aggataagaa gcatgaacgg catcccatct tcggtaatat cgtcgacgag
      421 gtggcctatc acgagaaata cccaaccatc taccatcttc gcaaaaagct ggtggactca
      481 accgacaagg cagacctccg gcttatctac ctggccctgg cccacatgat caagttcaga
      541 ggccacttcc tgatcgaggg cgacctcaat cctgacaata gcgatgtgga taaactgttc
      601 atccagctgg tgcagactta caaccagctc tttgaagaga accccatcaa tgcaagcgga
      661 gtcgatgcca aggccattct gtcagcccgg ctgtcaaaga gccgcagact tgagaatctt
      721 atcgctcagc tgccgggtga aaagaaaaat ggactgttcg ggaacctgat tgctctttca
      781 cttgggctga ctcccaattt caagtctaat ttcgacctgg cagaggatgc caagctgcaa
      841 ctgtccaagg acacctatga tgacgatctc gacaacctcc tggcccagat cggtgaccaa
      901 tacgccgacc ttttccttgc tgctaagaat ctttctga
//
