LOCUS       sequence-62286-e        1098 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Wu K, Lu G, Sehnke P, Ferl RJ
  TITLE     The heterologous interactions among plant 14-3-3 proteins and 
            identification of regions that are important for dimerization.
  JOURNAL   Arch Biochem Biophys. 1997 Mar 1;339(1):2-8.
  PUBMED    9056226
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Biochem Biophys."; date: "1997-03-1"; volume: "339(1)"; pages: "2-8"
COMMENT     Sequence Label: pET15b-At 14-3-3 upsilon
FEATURES             Location/Qualifiers
     source          1..1098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgtcttctg attcgtcccg ggaagagaat gtgtacttgg ccaagttagc cgagcaagct
      121 gagcgttacg aggaaatggt tgagttcatg gagaaagttg caaagaccgt ggagaccgag
      181 gaacttactg ttgaagagag gaatctcttg tctgttgctt acaagaacgt gattggtgct
      241 aggagagctt cttggaggat tatctcttcc attgagcaga aggaagatag caggggcaac
      301 agtgatcatg tttcgattat caaggattac agaggcaaga ttgaaactga gctcagcaag
      361 atttgtgatg gcattttgaa ccttcttgag gctcatctca ttcctgctgc ttctttggct
      421 gagtccaaag ttttttacct gaagatgaag ggagattatc atcggtacct tgctgaattc
      481 aagactggtg ctgagaggaa agaagctgct gagagcactc ttgttgccta caagtctgct
      541 caggatattg ctcttgctga tctggctccc actcacccaa tcagactggg gcttgctctt
      601 aacttctctg ttttctacta tgagattctc aactcatctg atcgtgcgtg tagtctcgca
      661 aagcaggctt ttgatgaggc aatctcggag ctagacacat tgggagagga atcatacaag
      721 gacagtacat tgatcatgca gcttctccgt gacaatctca ccctctggac ttctgacctc
      781 aatgacgaag ctggtgatga tatcaaggaa gccccgaaag aggtgcagaa agttgatgaa
      841 caagcccaac caccaccttc gcagtgataa aatcagatcc atggatgatt tgcagacaaa
      901 aaatatatgg ctgttctgtg tttttaacag aaaaaacctt gtagtttcta acatgggctg
      961 tagtttcnta ncatgaattt gtagtagtta ttgtagctgc atgaattgct atcgatgnta
     1021 aaaaaaaaan ccgaattccn cttgnnnccg ttgctacgnt cngtgtgcta cagccgaagg
     1081 aancgtgaat ggctgctg
//
