LOCUS       sequence-62287-e       15767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15767)
  AUTHORS   Cecena G, Wen F, Cardiff RD, Oshima RG
  TITLE     Differential sensitivity of mouse epithelial tissues to the 
            polyomavirus middle T oncogene.
  JOURNAL   Am J Pathol. 2006 Jan;168(1):310-20.
  PUBMED    16400032
REFERENCE   2  (bases 1 to 15767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Pathol."; date: "2006-01"; volume: "168(1)"; pages: "310-20"
COMMENT     Sequence Label: KFS2MT
FEATURES             Location/Qualifiers
     source          1..15767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(3043..3076)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3213..3347
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3467..3601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3716..3850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3872..3905)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7219..7791
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7242..7793
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7386..7403)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             8732..8749
                     /codon_start=1
                     /product="epitope tag from middle T antigen of mouse 
                     polyoma virus"
                     /label=Glu-Glu tag
                     /translation="EYMPME"
     promoter        complement(12907..12925)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12908..12925)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(13216..13235)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(13450..13469)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13604..13626
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(13675..13693)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13761..13865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13866..13934,13935..14726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14084..14103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14897..15485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15386..15405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15639..15656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     15737..15756
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15737..15755
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aattcgagct cgcccgggga tcctctagag tcgacctgca gcccaagctt agcaataaca
       61 gtaaaaggca gtacatagct tgttgactcc acatacttta ttataaaata ctgcccaact
      121 tgacagttct ggaatccagt gggggaatat aaaggtgaaa gcaggagaga cccctctgac
      181 tggaacctct tacctcccag aagccttgta tgcaaaacca gtgggcattc atttgtatgt
      241 tattttgcat cccgtttgcc tcccagcctt cagcaggccc cgaccctccc ctggccagct
      301 tccaccctga ctgccccctg gctggctccc attgagcact gtggggctct ccccaccatt
      361 aggtgacaga tcaggaacaa tccaggctca ggctctttat ctgtgctctg cctcccacct
      421 ggcaggtcca ctggccaggc ttttccaggg tcccttctct cccaggtctg ccctactatt
      481 tgtcctcccc ttccccctca gctggtagct cgataagaat caataggtcc actccagagc
      541 aaagaacaca gccaaatgtg tcataccagg ccctgccaga aaaacgagct gctggagctg
      601 acaaacttga aggccaaaca cctaagggtt ccccccaaca cttcattcag cagggatggt
      661 cattcagctt cagggggcag gcagcatgaa agcctcccta cctccatcct tctcacacag
      721 aggctgggga gagcatcttg gaggatgcag tcccctgggg ccaggcttct aatccagaca
      781 gcccttacaa ggggggacag gggaaggact ggcttggaga aaagtcctag aaaagagggg
      841 aggggcactg gccaccaggg ctgggtcgct gctatgatgg tcctaggagt gcctgcctgt
      901 cctctcaggc cccatgcgat gtaggacaca ttacttttat ttatttattt atttattttg
      961 agtcagagtt tcgctctggt tgcccaggct ggagcgcgac ggcacgatct tggctcactg
     1021 caacctctgc ctcctgggtt caagcgattc tcctgcctca gcctcctgag tagctgggat
     1081 tacaggcaca cactgtgctg gttaattttt gtatttttag tagagaaggg gtgtcaccat
     1141 gttggtcagg ctggtctcaa attttttttt tttttttttt ttttttttga gacagagtct
     1201 tgctctgttg tctaggctgg agtgcagtgg catcgaactc ttgacctcaa gtgatccacc
     1261 cgcctcggcc tcccaaagtg cttggattac aggcatgagc cactgtgccc ggcgatgtgg
     1321 gacacattat catctctgtg agagattttt ggtctctttt gtcaccgccc ttctctccca
     1381 gctcctagaa ctgggcctgg ctcacagtag gtgctgaatg catactggtt gaattgtaaa
     1441 tgctcaggat ttgtttaatt aaggatgcag gaaaggtgat ataccggtgt gcagaagtca
     1501 ggatgcattc cctgtccaaa tcacagtgtt ccactgaggc aaggcccttg ggagtgaggt
     1561 cgggagaggg gagggtggtg gagggggctc agagactggg tttgttttgg ggagtctgca
     1621 cctatttgct gagtgaatgt atgtgtgtgt gcatttgaga gcacacctct gtatgattcg
     1681 ggtgtgagtg tgtgtgagga aacgtgggca ggcgaggagt gtttgggagc caggtgcagc
     1741 tggggtgtga gtgtgtaagc aagcagctat gaggctgggc attgcttctc ctcctcttct
     1801 ccagctccca gcctttcttc cccgggactc ctggggctcc aggatgcccc caagatcccc
     1861 tccacaagtg gataatttgg gctgcaggtt aaggacagct agagggactc acaggccatt
     1921 ccacccgcac accaccagac ccccaaattt cttttttctt ttttttttga gacagagtct
     1981 cactctgtcg ccaggctgca gtggcgcgat ctcggctcac tgcaacctcc gcctcccagg
     2041 ttcaagcgat tccccttcct cagcctccca agtagctgag actacaggcg tgcaccatca
     2101 cgtccggcta attttttgta ttttagtaga gaggggtttc accatgttgg ctaggatggt
     2161 ctcgatctcc tgacctcgtg atccgcccac ctaggcctcc caaagtgctg agattacagg
     2221 cgtgagccac tgcgcccggt caagactccc aaatttcaaa ctcgccagca cctcctccac
     2281 ctgggggaga agagcataat aacgtcattt cctgccctga aagcagcctc gagggccaac
     2341 aacacctgct gtccgtgtcc atgcccggtt ggccaccccg tttctggggg gtgagcgggg
     2401 cttggcaggg ctgcgcggag ggcgcggggg tggggcccgg ggcggagcgg cccggggcgg
     2461 agggcgcggg ctccgagccg tccacctgtg gctccggctt ccgaagcggc tccggggcgg
     2521 gggcggggcc tcactctgcg atataactcg ggtcgcgcgg ctcgcgcagg ccgccaccgt
     2581 cgtccgcaaa gcctgagtcc tgtcctttct ctctccccgg acagcatgag cttcaccact
     2641 cgctccacct tctccaccaa ctaccggtcc ctgggctctg tccaggcgcc cagctacggc
     2701 gcccggccgg tcagcagcgc ggccagcgtc tatgcaggcg ctgggggctc tggttcccgg
     2761 atctccgtgt cccgctccac cagcttcagg ggcggcatgg ggtccggggg cctggccacc
     2821 gggatagccg ggggtctggc aggaatggga ggcatccaga acgagaagga gaccatgcaa
     2881 agcctgaacg accgcctggc ctcttacctg gacagagtga ggagcctgga gaccgagaac
     2941 cggaggctgg agagcaaaat ccgggagcac ttggagaaga agggacccca ggtcagagac
     3001 tggagccatt acttcaagat catcgaggac ctgagcaagc ttataacttc gtatagcata
     3061 cattatacga agttatgagc ttgcagatcg atctgcgact ctagaggatc ataatcagcc
     3121 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     3181 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     3241 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3301 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctgc gactctagag
     3361 gatcataatc agccatacca caattggtag aggtttactt gctttaaaaa acctcccaca
     3421 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     3481 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3541 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     3601 cttctagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     3661 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3721 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3781 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3841 tgtctggatc cccgggtacc gagctcgaat tataacttcg tatagcatac attatacgaa
     3901 gttatcgaat tcgctgaggg ctcaggtaag gggtaggagg gacctcaact cccagccttg
     3961 tctgaccctc caattataca ctcctttgcc tctttccgtc attccataac caccccaacc
     4021 cctactccac cgggaggggg ttgggcatac ctggatttcc atccgcgcac ctagccacag
     4081 ggtccctaag agcagcagca gctaggcatg ggagggctct ttcccaggag agagggggaa
     4141 ggggacaggg ttgagagctt tacagaggaa gtggacagca tggagggagg taaggaaagg
     4201 cctgtaaaga ggaggagaca ctggctctgg cggaatgggg actattggag ggttaagcgg
     4261 atgtggctaa ggctgagtca tctaggagta aacaagaggc cttcctttgg gaggagccaa
     4321 tccagggtgt agggggccca gagtgaccag gtgcactagg gaaaaaatgc caggagaggg
     4381 ccaggaagag gacttgttag tagcgactca cttctgggca ggcaggccag ccagctagcc
     4441 agcctgctga ggcttcccaa gaggggcaga gtgctgggat ctgggaatcc aggaaaggag
     4501 ggaatggggt ggggctagat gaaaagggat aggtgtccag ggagagcctc tggctattcc
     4561 tgggaccagg aagttttcac taggatacat aacacttttt acacactcac cccacccatc
     4621 cctggctttc tattcatgga acaacctctc tctacaatcc ctccagatct tcgcaaatac
     4681 tgtggacaat gcccgcatcg ttctgcagat tgacaatgcc cgtcttgctg ctgatgactt
     4741 tagagtcaag taagtttggg ggctagagag ctgggggtcc aggggtggag ctaagaagga
     4801 ctgctcccca ggctgggtag ttaggggcac acagtgggat cctgttaggt gtgggtggat
     4861 gagagtcagg gtccatcagt gtattcattt aactgttcat ttgtataacc ccgtttaaga
     4921 atactgtcct ccaagtgcca agaatggtgc tcaggggatt accacctaat tgctgactca
     4981 agttgctggt ttgcaatggg cacagaactt ctcttagtag gtggcatgag ttgagaaggt
     5041 tctggatcag agataggggc ccctctgatc acctccactc ctataggtat gagacagagc
     5101 tggccatgcg ccagtctgtg gagaacgaca tccatgggct ccgcaaggtc attgatgaca
     5161 ccaatatcac acgactgcag ctggagacag agatcgaggc tctcaaggag gagctgctct
     5221 tcatgaagaa gaaccacgaa gaggcaagca ggggccactg gccaggccag ggattgaggg
     5281 gccaagagaa gtctgggtcg gagaatagac aagacaaacc aactgcaagt agccttgcta
     5341 agacgtttag aaatagcagc ctgggctctt cttaaataag accgttctga tgaagagcat
     5401 tctcaggggg tcgagtacac cctggctcac ctgaattaca ggtcaaaatg atatgggtta
     5461 gaaaatgaga tgagaacaga agtagaagca gctaacacta ggagctgggg gtgataaagg
     5521 aatgacagca gatggagttg gcagcttcct aaaagatggt agaaggagca ggtttgtgaa
     5581 ggggagggtg gataaaggaa cagggtgaag ttacagagaa accatcagtg agtgggtggc
     5641 atttctaccc actggagtag aaaggccaga actggcattg ccctgagtgc aagccaaggg
     5701 gttcctcctg tctcttctcc aactgtaggc ctcctagaag aggcaatcac agaagaaagg
     5761 ccttgttgga gctctgaccc tgaaccctcc tcacttttgc ccctgtcacc tttaggaagt
     5821 aaaaggccta caagcccaga ttgccagctc tgggttgacc gtggaggtag atgcccccaa
     5881 atctcaggac ctcgccaaga tcatggcaga catccgggcc caatatgacg agctggctcg
     5941 gaagaaccga gaggagctag acaagtactg gtctcagcag gtgcgtgagg ggaggggatg
     6001 gctgccaagg tgtgggaggg aggcagacgg aatgaggggc ctgatggact gtccccatcc
     6061 tgcagattga ggagagcacc acagtggtca ccacacagtc tgctgaggtt ggagctgctg
     6121 agacgacgct cacagagctg agacgtacag tccagtcctt ggagatcgac ctggactcca
     6181 tgagaaatct ggtgagtgcc ttcacatcac ctgcccagct cctccttcac ttggcctcag
     6241 acccaaccct gtctcaaccc aaatcctatc cctcatatca tgagttcctt tagctcagaa
     6301 agagtcagtt tcctctttgc atttccctcc actcctatcc cttatcccag tacttggcac
     6361 atagcaggtg cccaaaaaag tttccaaaag tgaagggatg agcagtcctg ggactctggg
     6421 ctcaccctgc ccctcctctc tgtgcccctg cagaaggcca gcttggagaa cagcctgagg
     6481 gaggtggagg cccgctacgc cctacagatg gagcagctca acgggatcct gctgcacctt
     6541 gagtcagagc tggcacagac ccgggcagag ggacagcgcc aggcccagga gtatgaggcc
     6601 ctgctgaaca tcaaggtcaa gctggaggct gagatcgcca cctaccgccg cctgctggaa
     6661 gatggcgagg actttaagtg agtggggctc tcctacccac acgtgctggg atcaggagat
     6721 cacttctccc caaagtctga gcttttggaa gcaccccatg tgtctgttca ctggtatcca
     6781 ctgagcactg ggccgttgct ccgtgggtgc tcctgtgtct tcaagggagt aacagttaca
     6841 gaggtctccc ccttgaagaa agcaaactaa gtattgtccc tagctgtact tagtatgcaa
     6901 atgaagtttg gccttgagtt tcccttttct ggaggaagag gctgagggtg atttggagat
     6961 aaaggtagag gtcaggaggc tttttccctc tacctttctt gtctcccttc tactccacgg
     7021 ggctgtttat aacttgggct tggtcttctg ttacagtctt ggtgatgcct tggacagcag
     7081 caactccatg caaaccatcc aaaagaccac cacccgccgg atagtggatg gcaaagtggt
     7141 gtctgagacc aatgacacca aagttctgag gcattaagcc agcagaagca gggtaccccg
     7201 cccccactag tccgcgggcc cctctccctc ccccccccct aacgttactg gccgaagccg
     7261 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     7321 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     7381 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     7441 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     7501 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     7561 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     7621 ctcaagcgta gtcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     7681 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     7741 aggccccccg aaccgcgggg acgtggtttt cctttgaaaa cacgatgata atatggatag
     7801 agttctgagc agagctgaca aagaaaggct gctagaactt ctaaaacttc ccagacaact
     7861 atggggggat tttggaagaa tgcagcaggc atataagcag cagtcactgc tactgcaccc
     7921 agacaaaggt ggaagccatg ccttaatgca ggaattgaac agtctctggg gaacatttaa
     7981 aactgaagta tacaatctga gaatgaatct aggaggaacc ggcttccagg taagaaggct
     8041 acatgcggat gggtggaatc taagtaccaa agacaccttt ggtgatagat actaccagcg
     8101 gttctgcaga atgcctctta cctgcctagt aaatgttaaa tacagctcat gtagttgtat
     8161 attatgcctg cttagaaagc aacatagaga gctcaaagac aaatgtgatg ccaggtgcct
     8221 agtacttgga gaatgttttt gtcttgaatg ttacatgcaa tggtttggaa caccaacccg
     8281 agatgtgctg aacctgtatg cagacttcat tgcaagcatg cctatagact ggctggacct
     8341 ggatgtgcac agcgtgtata atccaaaacg gcggagcgag gaactgagga gagcggccac
     8401 agtccactac acgatgacta ctggtcattc agctatggaa gcaagtactt cacaagggaa
     8461 tggaatgatt tcttcagaaa gtgggacccc agctaccagt cgccgcctaa gactgccgag
     8521 tcttctgagc aacccgacct attctgttat gaggagccac tcctatcccc caacccgagt
     8581 tctccaacag atacacccgc acatactgct ggaagaagac gaaatccttg tgttgctgag
     8641 cccgatgaca gcatatcccc ggaccccccc agaactcctg tatccagaaa gcgaccaaga
     8701 ccagctggag ccactggagg aggaggagga ggagtacatg ccaatggagg atctgtattt
     8761 ggacatccta ccgggggaac aagtacccca gctcatcccc ccccctatca ttcccagggc
     8821 gggtctgagt ccatgggagg gtctgattct tcgggatttg cagagggctc atttcgatcc
     8881 gatcctagat gcgagtcaga gaatgagagc tactcacaga gctgctctca gagctcattc
     8941 aatgcaacgc cacctaagaa ggctagggag gaccctgctc ctagtgactt tcctagcagc
     9001 cttactgggt atttgtctca tgctatttat tctaataaaa cgttcccggc atttctagta
     9061 tacgcggccg cgaggtaccc tttgggagca ggaggccaat aaaaagttca gagttcattg
     9121 gatgtcactt tgtcttcttt tggctgtttt cattgtgcac aaatgcccta acccaacagt
     9181 cccatccctg atccagcaga aaccacctct gacccctgag gtttcatata gattggggtg
     9241 tagaaggaag agggatctgt attcttggaa acacttctga gagacagagg agggagcagt
     9301 agatgtgatg ggtcacaggc tgtgggatcc ctacaaggga actgtggaat ctttgccttg
     9361 aagtgatctg gtgttcaaaa ggtccctgac tgatcattag cagaaatctg aaccagagga
     9421 ggaaattagg ccaaagacag aggacagagg agacagggag acacatctgg ccctccccta
     9481 ccctgctgct ttatnccctt acaccactgc ccagggcttc ccacccacag tttttnnctc
     9541 caaccccagg ccatactcat cctccaaacc tatggtgacc ctcaactctg ttcattcatc
     9601 taacggagat atgattttta gggtgctaga ggtctggcta gaagttgagt aaatgatcat
     9661 ggaagttccc atctaggaaa gaggtgcccc tgagtaagca cccgtatagg taaggatggg
     9721 catcttaaaa ctaaccgagg ctgggcacag tggctcacat ccgtaatccc agcagtgcca
     9781 ccacacccgg ctaatttttn aatttttcat agagttggga tttcactata ttgcccaggc
     9841 tggtctggaa ctcctggcct caagtgatcc ncccgcttgt accctttgga gtgattggat
     9901 gccactttgt gtcttccttn gctgttttca tacttgtgtg aaaatnccct aacccaacat
     9961 gattacatgt agatattcgt tgcagcagtt ttccaaaaca aaactactaa agatcaangc
    10021 acccatcact caaagaccta ggttaggtna attttttttt tttttttttt tttttttttt
    10081 gaggcagagt cttgctctgt cacccaggct ggagtgcagt ggcgcgatcc gggctcactg
    10141 caactgcctc ctgggttcga gggattcttc tgcctcagcc tcctgagtag ctgggatttc
    10201 aggggcccgc caccatgcct ggctaatttt tgtattttta gtagagacgg ggtgtcacct
    10261 tgttggccag gctggtctcg aactcctgac ctcgtgatcc gcccnctcgg cctcccaaaa
    10321 tgcatggata tacacggtga gtcaccacgc ccggcctagg ttaattttta aaatgttggg
    10381 ttaaggcaaa aaaaaatttt ttaatttaaa aattagccag gcatggtgac atgtacctgt
    10441 agtcccactt aatcaggagg ctgaggtgga aggattgctt gagtccagga agtaaaggtt
    10501 gcagtaagct gtgatcatgc cactgcattt cagcctaggt aacagagtgt gactctgttt
    10561 cacaaaaaaa agctggccgg gcacagnnca cgcctgtaat cccagcaatt tgggaggccg
    10621 aggcgggcag atcacaatgt caggagattg agcgatcctg gctaacatgg tgaaactctg
    10681 tctctattaa aagtacaaaa aattagccag gcagctgggt gtggtggctc atncctgtta
    10741 tnccagcact ttgggaggcc aaggcgggcg gataacttga ggtcaggagt tcgagaccag
    10801 cctggccaac atggtgaaac ctctgtctct actaaaaata caaaattagc tgggtgtggt
    10861 ggcacatgcc tataatccca gctacttggg aagttgaggc aggagaatcg cttgaaccca
    10921 ggaggtggag gttgcaataa gccgagatca tgccattgca ctccagcctg ggcaacaaga
    10981 gcgaaactct gtctcaaaaa aaaatatata tataatatat atatgtgtgt gtatgtatat
    11041 atgtgtgtat atatgtatat atgagtatat gtgtatatat gtgtatatgt gtatatacat
    11101 atgtgcatat atatgtgtgt atatgtgtgt atgtatatat atacatatat acatacatat
    11161 atatatatat acatacacac atacacacac acacacatat atatgaaata aaatttaaaa
    11221 tcttgctggc atggtntnat gcctgtaatc ccagnanntt gggaggccaa ggtgggtggt
    11281 nntctgaggt tgggagtgga gaaactctgt ctctactaaa aatacaaaat tagccaggcg
    11341 tgatggcgca tgattgtaat cctatctact tgggagactg aggcaggaga atcgcttgaa
    11401 ccaaggagtt ggaggttgca gtgagacgag atcgagccac tgcactccag cctggcaaca
    11461 gagcaagact ctgtctcaaa aaaaaaaaaa aaaaaaatag ccatgcgtag tggcatgacg
    11521 acctgtagtc ccagctactt gggaggctga ggcaggagat ttgcttgnag cccagcaggc
    11581 agaggttgca gtgagtgaag atcgcgccac tgtacttcng nctgggtgac agaacaagac
    11641 tccatctcaa ataaataaat aaataaaact aaataaaata aaatctcccc tctccactcc
    11701 ctagaagtaa ccactgttaa atacctttta aaattagaca tatacaagca catttcatta
    11761 tgttttatat atgtaaaatt atccagctct gcatcttgat ttcttttttt attttttttt
    11821 aattgagaca gagtctcact ctgtcgccca ggctggagtg cagtggcacc atcacggctc
    11881 actgccacct ctgcttcccg ggccctcaag cgattctcct gcctcagcct actgagtagc
    11941 tgggattacc aggcacccac caccacgcct ggctaattgt tgtattttta gtagagacag
    12001 ggtttcacca tgttgatcag gctggtctcg aactcctgac cttgtgatcc acctgcctcg
    12061 gcctcccaaa gtgctgggat tacaggcgtg nnccatgctt ggctttgatt tcttaaattc
    12121 tactttcttc atttaacaat acgtctcggg gctgggcatg gtggctcatg ctgtaatccc
    12181 agcactttgg gaggctgagg cggacagatc acgagatcag gagatcgaga ctatcctggc
    12241 taacacagtg gaaccccatc tctactaaaa atgcaaaaaa aattagctgg gcatggtggc
    12301 gggcacctgt agtcccagct actctggagg ctgaagcagg agaatggaat gaacctggaa
    12361 ggcggagctt gcagtgagcc gagattccac cactgcattc cagcctgggc aacagagcaa
    12421 gactctgtct caaaaaataa aaataaatga aaatacatct tggacacctt tctgaatcag
    12481 tatacataga cctgccccat tcatttgagt agctgcttag tactctactg cgtggagtac
    12541 aaattaatta acctaaccta ggtctttaag taatggatgt cttgatcttt tttttttttt
    12601 tttttttttt tttgagacag aatctcactc tgtcgcccag gctggagtac agtgctatga
    12661 tctcagctca ctgcaacctc tccctcctgg gttcaagtaa ttctcctggc tcagcctccc
    12721 gagtagctgg gactacaggt gcatgccacc atgcccagct aatttttagt attttagtag
    12781 agacagggtt tcaccgtgtt gccaaggctg gcctcgaact gagctcaggc aatccgccag
    12841 ccttggcctc ccaaagtact ggnattacag gcatgagcaa ccacgcccag cctaaaaagc
    12901 ttgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt
    12961 gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa
    13021 gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt
    13081 ctgagtttct ggtaacgccg ttccgcaccc cggaaatggt caccgaacca atcagcaggg
    13141 tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
    13201 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
    13261 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
    13321 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
    13381 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgatatggtg
    13441 cactctcagt acaatctgct ctgatgccgc atagttaagc caactccgct atcgctacgt
    13501 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
    13561 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
    13621 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc ttgaagacga aagggcctcg
    13681 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
    13741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    13801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    13861 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    13921 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    13981 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    14041 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    14101 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    14161 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    14221 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    14281 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    14341 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    14401 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    14461 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    14521 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    14581 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    14641 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    14701 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    14761 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    14821 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    14881 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    14941 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    15001 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
    15061 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    15121 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    15181 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    15241 gcttggagcg aacgacctac accgaactga gatacctaca gcgcgagcat tgagaaagcg
    15301 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    15361 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    15421 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    15481 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    15541 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    15601 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    15661 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    15721 gctggcttat cgaaattaat acgactcact atagggagac cggaatt
//
