LOCUS       sequence-62299-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Peters RT, Toby G, Lu Q, Liu T, Kulman JD, Low SC, Bitonti AJ, 
            Pierce GF
  TITLE     Biochemical and functional characterization of a recombinant 
            monomeric Factor VIII-Fc fusion protein.
  JOURNAL   J Thromb Haemost. 2012 Dec 4. doi: 10.1111/jth.12076.
  PUBMED    23205847
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Thromb 
            Haemost. 2012 Dec 4. doi: 10.1111/jth.12076."
COMMENT     Sequence Label: pSYN-Kex-002
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    22..143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(33..52)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     87..106
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(301..699)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(718..765)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 gaggtgaggt atcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
       61 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      121 ttataagctg caataaacaa gttggggtgg gcgaagaact ccagcatgag atccccgcgc
      181 tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag
      241 gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct gctcctcggc cacgaagtgc
      301 ttagccctcc cacacataac cagagggcag caattcacga atcccaactg ccgtcggctg
      361 tccatcactg tccttcacta tggctttgat cccaggatgc agatcgagaa gcacctgtcg
      421 gcaccgtccg caggggctca agatgcccct gttctcattt ccgatcgcga cgatacaagt
      481 caggttgcca gctgccgcag cagcagcagt gcccagcacc acgagttctg cacaaggtcc
      541 cccagtaaaa tgatatacat tgacaccagt gaagatgcgg ccgtcgctag agagagctgc
      601 gctggcgacg ctgtagtctt cagagatggg gatgctgttg attgtagccg ttgctctttc
      661 aatgagggtg gattcttctt gagacaaagg cttggccatg gtttagttcc tcaccttgtc
      721 gtattatact atgccgatat actatgccga tgattaattg tcaacacgtg ctgatcagat
      781 ccgaaaatgg atatacaagc tcccgggagc tttttgcaa
//
