LOCUS       sequence-62303-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  TITLE     OPPF-UK pOPIN vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOPINE-3C-CD4
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     96..115
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    114..169
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     215..234
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(324..341)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttagaggccc caaggggtta tgctagttac atatgggcat atgttgccaa actctaaacc
       61 aaatactcat tctgatgttt taaatgattt gccctcccat atgtccttcc gagtgagaga
      121 cacaaaaaat tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaagtc
      181 agatgctcaa ggggcttcat gatgtcccca taatttttgg cagagggaaa aagatcgatc
      241 tcagtggtat ttgtgagcca gggcattggc cacaccagcc accaccttct gataggcagc
      301 ctgcacctga ggttaatcac ttagtgatgg tgatggtgat gtttattcaa ccctttggat
      361 aaaacctgga tcttggagtc catcttgacc tcttcacctt cactcagtag acattgccac
      421 acccctgctt caggggcctg cacttgaatc actttctcct gcctggagac cctggcctcc
      481 tgattctcct gcttcaagat cagtctcatc ttgggtgagg tgggtcccat cacctcacag
      541 gtcaaagtgt tgctgtcggg ctgagtcact ttcatcacca ccaggttcac ttcctgatac
      601 agtatccctc tgtccagagt cagggtcagg ttgccagaac cagcaaactg aagggagacc
      661 tggggtatct gaagggtgag tgggagcgtt tcggacagct ggaacttggg gttgctagta
      721 gacttctgca cagacacctt ttggttcttt agggagaagg tgatccag
//
