LOCUS       sequence-62304-e         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  TITLE     OPPF-UK pOPIN vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOPINE-3C-HALO7
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     103..122
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    121..176
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     222..241
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(331..348)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gacccgttta gaggccccaa ggggttatgc tagttacata tgggcatatg ttgccaaact
       61 ctaaaccaaa tactcattct gatgttttaa atgatttgcc ctcccatatg tccttccgag
      121 tgagagacac aaaaaattcc aacacactat tgcaatgaaa ataaatttcc tttattagcc
      181 agaagtcaga tgctcaaggg gcttcatgat gtccccataa tttttggcag agggaaaaag
      241 atcgatctca gtggtatttg tgagccaggg cattggccac accagccacc accttctgat
      301 aggcagcctg cacctgaggt taatcactta gtgatggtga tggtgatgtt tcagagtaga
      361 cagccagcgc gcgatctcgc tgccgatcag gtccgggttg tcttcttgca gcagattcag
      421 acccgggccg atgtccacag ccttgcagtt aggcaggctt ttggccaggc gagcggcttc
      481 ggccggtggg atcagaacgc ctggggtgcc ccagaacagc agcttcggga caggggactg
      541 gtgcagccag tccatgtatt cttcgaccag cgcgacgatg ttcgctggct caccggcgat
      601 tggcagctcg tttgggaagc gccacagtgg ctcgcggtca acaggattca ggaacggctc
      661 gcggtaatgg tccatctcga cttcagtcag cgggcggacg acacccatcg gcagcgtacc
      721 ctcgataaaa acgttctgat cgatgatcag cttgcggccg acgtcggtgg tgcggaaggc
      781 ctgg
//
