LOCUS       sequence-62306-e         533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 533)
  TITLE     OPPF-UK pOPIN vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pOPINE-3C-eGFP
FEATURES             Location/Qualifiers
     source          1..533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     104..123
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    122..177
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     223..242
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(332..349)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(395..416)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agacccgttt agaggcccca aggggttatg ctagttacat atgggcatat gttgccaaac
       61 tctaaaccaa atactcattc tgatgtttta aatgatttgc cctcccatat gtccttccga
      121 gtgagagaca caaaaaattc caacacacta ttgcaatgaa aataaatttc ctttattagc
      181 cagaagtcag atgctcaagg ggcttcatga tgtccccata atttttggca gagggaaaaa
      241 gatcgatctc agtggtattt gtgagccagg gcattggcca caccagccac caccttctga
      301 taggcagcct gcacctgagg ttaatcactt agtgatggtg atggtgatgt ttcttgtaca
      361 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
      421 cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg
      481 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcg
//
