LOCUS       sequence-62311-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a catalytically inactive, human codon-optimized 
            Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR
            promoter for mammalian gene knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim 
            WA
  TITLE     Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific 
            Control of Gene Expression.
  JOURNAL   Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
  PUBMED    23452860
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.02"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1173-83"
COMMENT     Sequence Label: pdCas9::BFP-humanized
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..37)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gctcgctgga actacctacc ttgcgctttt tcttgggagc tccctcatcc cctccgagct
       61 gtgagaggtc aatccgagtt tcgtaaagtc cggtgatgct ctggtggatg agggtggcgt
      121 ccagcacttc tttggtggag gtatagcgtt tccggtcgat ggtggtgtcg aagtacttga
      181 atgcggcagg ggcaccaaga ttggtgaggg tgaacaggtg gatgatattc tcagcctgct
      241 cccgaattgg tttgtctctg tgcttattgt atgcgctaag gactttatcg aggttggcat
      301 cagcgagaat gacgcgttta gaaaactcgg aaatctgctc aatgatctcg tccagataat
      361 gcttatgctg ctccacaaac agttgctttt gctcgttgtc ctcggggctc cccttcagct
      421 tctcgtaatg gcttgcaagg tagaggaaat tcacgtactt ggaggggaga gccagctcat
      481 tacccttctg aagttcgcca gcggaggcga gcatccgctt ccgcccattc tccagttcga
      541 acaggctata ctttgggagc ttgatgataa ggtccttctt gacttccttg taacctttgg
      601 cttcgagaaa gtcaattggg ttcttctcga aggagctgcg ctccataatg gtgataccca
      661 gcagttcctt cacgctcttg agcttcttag actttccttt ttccacctta gccacgacca
      721 ggacagaata agccacagtt ggtgaatcga atccgccgta cttcttaggg tcccaatcct
      781 tcttccttgc aatgagcttg tcggagttgc gcttggggag gattgattcc ttagaaaagc
      841 ctccggtttg cacctcggtt ttcttcacga tgttcacttg aggcatagaa agcactttgc
      901 gcacggtagc gaaatctctg cccttgtcc
//
