LOCUS       sequence-62339-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   D'Alessio A, Kluger MS, Li JH, Al-Lamki R, Bradley JR, Pober JS
  TITLE     Targeting of tumor necrosis factor receptor 1 to low density plasma 
            membrane domains in human endothelial cells.
  JOURNAL   J Biol Chem. 2010 Jul 30;285(31):23868-79. doi: 
            10.1074/jbc.M110.122853. Epub 2010 May 28.
  PUBMED    20511226
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.122853"; journalName: "J Biol Chem"; date: 
            "2010-07-30- 30"; volume: "285"; issue: "31"; pages: "23868-79"
COMMENT     Sequence Label: pBMNZ-neo-Flag-TNFR1 L380A
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     311..327
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             483..506
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa
       61 gagtttacta acagcccctt ctctccaagc tcacttacag gctctctact tagtccagca
      121 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
      181 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
      241 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
      301 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
      361 atcctctaga ctgccggatc cactagtaac ggccgccagt gtgctggaat tcgcccttca
      421 tgagggcctg gatcttcttt ctcctttgcc tggccgggag ggctctggca gccccactag
      481 ccgactacaa ggacgacgat gacaagctag ccctggtccc tcacctaggg gacagggaga
      541 agagagatag tgtgtgtccc caaggaaaat atatccaccc tcaaaataat tcgatttgct
      601 gtaccaagtg ccacaaagga acctacttgt acaatgactg tccaggcccg gggcaggata
      661 cggactgcag ggagtgtgag agcggctcct tcaccgcttc agaaaaccac ctcagacact
      721 gcctcagctg ctccaaatgc cgaaaggaaa tgggtcaggt ggagatctct tcttgcacag
      781 tggaccggga caccgtgtgt ggctgcagga agaaccagta ccggcattat tggagtgaaa
      841 accttttcca gtgcttcaat tgcagt
//
