LOCUS       sequence-62343-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Hafner M, Max KE, Bandaru P, Morozov P, Gerstberger S, Brown M, 
            Molina H, Tuschl T
  TITLE     Identification of mRNAs bound and regulated by human LIN28 proteins 
            and molecular requirements for RNA recognition.
  JOURNAL   RNA. 2013 May;19(5):613-26. doi: 10.1261/rna.036491.112. Epub 2013 
            Mar 12.
  PUBMED    23481595
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.036491.112"; journalName: "RNA"; date: "2013-05"; 
            volume: "19"; issue: "5"; pages: "613-26"
COMMENT     Sequence Label: pFRT/FLAG/HA-DEST LIN28A
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             81..104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             105..131
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    147..171
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(805..829)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccggact ctagcgttta
       61 aacttaagct tggtaccatg gactacaagg acgacgatga caagtaccct tatgacgtgc
      121 ccgattacgc tttgaaccag atatcaacaa gtttgtacaa aaaagcaggc ttcatgggct
      181 ccgtgtccaa ccagcagttt gcaggtggct gcgccaaggc ggcagaagag gcgcccgagg
      241 aggcgccgga ggacgcggcc cgggcggcgg acgagcctca gctgctgcac ggtgcgggca
      301 tctgtaagtg gttcaacgtg cgcatggggt tcggcttcct gtccatgacc gcccgcgccg
      361 gggtcgcgct cgacccccca gtggatgtct ttgtgcacca gagtaagctg cacatggaag
      421 ggttccggag cttgaaggag ggtgaggcag tggagttcac ctttaagaag tcagccaagg
      481 gtctggaatc catccgtgtc accggacctg gtggagtatt ctgtattggg agtgagaggc
      541 ggccaaaagg aaagagcatg cagaagcgca gatcaaaagg agacaggtgc tacaactgtg
      601 gaggtctaga tcatcatgcc aaggaatgca agctgccacc ccagcccaag aagtgccact
      661 tctgccagag catcagccat atggtagcct catgtccgct gaaggcccag cagggcccta
      721 gtgcacaggg aaagccaacc tactttcgag aggaagaaga agaaatccac agccctaccc
      781 tgctcccgga ggcacagaat tgacacccag ctttcttgta caaagtggtt gatatccagc
      841 acagtggcgg ccgctcaagt ctagagggcc cgcggttcga a
//
