LOCUS       sequence-62378-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty backbone for blue-light-regulated expression of a gene of 
            interest. Expression is repressed under blue illumination..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Ohlendorf R, Vidavski RR, Eldar A, Moffat K, Moglich A
  TITLE     From dusk till dawn: one-plasmid systems for light-regulated gene 
            expression.
  JOURNAL   J Mol Biol. 2012 Mar 2;416(4):534-42. doi: 
            10.1016/j.jmb.2012.01.001. Epub 2012 Jan 8.
  PUBMED    22245580
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2012.01.001"; journalName: "J Mol Biol"; date: 
            "2012-03-2- 2"; volume: "416"; issue: "4"; pages: "534-42"
COMMENT     Sequence Label: pDusk
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(72..104)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(108..125)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(135..152)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             171..193
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(522..541)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga cggagctcga
       61 attcggatcc gacccatttg ctgtccacca gtcatgctag ccatatggct gccgcgcggc
      121 accaggccgc tgctgtgatg atgatgatga tggctgctgc ccatggtata tctccttctt
      181 aaagttaaac aaaattattt ctagactccg ttgtgatgac gcattggtac gcggtatcgg
      241 gaggttcgaa aatttcgagc gatatcttaa ggggggtgcc ttacgtagaa ccccgtaggt
      301 catgcccgag gccggtcctg gatggcgcgg cggatacgct tgagcaggtt ttcgtcgaga
      361 agcggcttca aaaccacgtc ttttacgccg gcctcggcgg cccgggtcga gatgttttcg
      421 tccggatagc cggtgatcag gatcacgggc gtagatctcg atcctctacg ccggacgcat
      481 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
      541 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
      601 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
      661 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
      721 gcataaggga gagcgtcgag atcccggaca ccatcgaatg gtgcaaaacc tt
//
