LOCUS       sequence-62382-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Crook NC, Freeman ES, Alper HS
  TITLE     Re-engineering multicloning sites for function and convenience.
  JOURNAL   Nucleic Acids Res. 2011 Aug;39(14):e92. doi: 10.1093/nar/gkr346. 
            Epub 2011 May 17.
  PUBMED    21586584
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-08"; volume: "39(14):e92. doi"; pages: " 
            10.1093/nar/gkr346. Epub 2011 May 17"
COMMENT     Sequence Label: pTEF2
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(489..507)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      519..708
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 ccttttttac tcttccagat tttctcggac tccgcgcatc gccgtaccac ttcaaaacac
       61 ccaagcacag catactaaat ttcccctctt tcttcctcta gggtgtcgtt aattacccgt
      121 actaaaggtt tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg
      181 caataaaaat ttttatcacg tttctttttc ttgaaaattt tttttttgat ttttttctct
      241 ttcgatgacc tcccattgat atttaagtta ataaacggtc ttcaatttct caagtttcag
      301 tttcattttt cttgttctat tacaactttt tttacttctt gctcattaga aagaaagcat
      361 agcaatctaa tctaagttta ccccatcgat cacccactag ttctagaccc ccgaattccc
      421 cccaagcttt aaagtcgacc ccccctcgag accccggatc ccccgggctg caggatatca
      481 ttagttatgt cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa
      541 ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta
      601 agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt
      661 aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc
      721 ggccgg
//
